254565

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4172669..4173245 Replicon chromosome
Accession NZ_CP102947
Organism Escherichia coli strain SCAID WND1-2022 (119)

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag NW339_RS20535 Protein ID WP_001295743.1
Coordinates 4172958..4173245 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag NW339_RS20530 Protein ID WP_000063148.1
Coordinates 4172669..4172971 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW339_RS20515 (4169265) 4169265..4171415 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
NW339_RS20520 (4171465) 4171465..4171668 + 204 WP_000467859.1 YbdD/YjiX family protein -
NW339_RS20525 (4171679) 4171679..4172635 + 957 WP_001295745.1 GTPase -
NW339_RS20530 (4172669) 4172669..4172971 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
NW339_RS20535 (4172958) 4172958..4173245 - 288 WP_001295743.1 BrnT family toxin Toxin
NW339_RS20540 (4173442) 4173442..4174608 + 1167 WP_000800831.1 restriction endonuclease -
NW339_RS20545 (4174672) 4174672..4176291 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
NW339_RS20550 (4176281) 4176281..4176985 + 705 Protein_4028 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T254565 WP_001295743.1 NZ_CP102947:c4173245-4172958 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT254565 WP_000063148.1 NZ_CP102947:c4172971-4172669 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References