Detailed information of TA system TA254563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3795078..3796767
• Replicon: chromosome
• Accession: NZ_CP102947
• Organism: Escherichia coli strain SCAID WND1-2022 (119)

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: NW339_RS18715
• Protein ID: WP_000365807.1
• Coordinates: 3795078..3795998 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NW339_RS18720
• Protein ID: WP_001295795.1
• Coordinates: 3795985..3796767 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NW339_RS18675 (3790551)	3790551..3791261	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
NW339_RS18680 (3791568)	3791568..3791897	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
NW339_RS18685 (3791909)	3791909..3792075	-	167	Protein_3666	tail fiber assembly protein	-
NW339_RS18690 (3792146)	3792146..3792760	-	615	WP_001019923.1	YagU family protein	-
NW339_RS18695 (3792975)	3792975..3793160	-	186	WP_000146240.1	hypothetical protein	-
NW339_RS18700 (3793209)	3793209..3793367	+	159	Protein_3669	integrase	-
NW339_RS18705 (3793594)	3793594..3793929	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
NW339_RS18710 (3794137)	3794137..3794913	+	777	WP_000171155.1	site-specific integrase	-
NW339_RS18715 (3795078)	3795078..3795998	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NW339_RS18720 (3795985)	3795985..3796767	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
NW339_RS18725 (3797051)	3797051..3800149	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3759474..3803830	44356

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T254563 WP_000365807.1 NZ_CP102947:c3795998-3795078 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT254563 WP_001295795.1 NZ_CP102947:c3796767-3795985 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0