25456

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1577394..1578049 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag MAF_RS07420 Protein ID WP_003407268.1
Coordinates 1577394..1577795 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag MAF_RS07425 Protein ID WP_003407272.1
Coordinates 1577792..1578049 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS07405 1572866..1574251 - 1386 WP_003407260.1 cytochrome P450 -
MAF_RS07410 1574329..1575363 + 1035 WP_003407264.1 AraC family transcriptional regulator -
MAF_RS07415 1575409..1577139 - 1731 WP_013988836.1 PE family protein -
MAF_RS07420 1577394..1577795 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
MAF_RS07425 1577792..1578049 - 258 WP_003407272.1 ribbon-helix-helix domain-containing protein Antitoxin
MAF_RS07430 1578132..1579091 - 960 WP_003407276.1 alpha/beta hydrolase -
MAF_RS07435 1579116..1580078 - 963 WP_003407279.1 alpha/beta hydrolase -
MAF_RS07440 1580077..1580814 + 738 WP_031647000.1 lysoplasmalogenase -
MAF_RS07445 1580895..1582862 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T25456 WP_003407268.1 NC_015758:c1577795-1577394 [Mycobacterium tuberculosis variant africanum GM041182]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T25456 NC_015758:c1577795-1577394 [Mycobacterium tuberculosis variant africanum GM041182]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT25456 WP_003407272.1 NC_015758:c1578049-1577792 [Mycobacterium tuberculosis variant africanum GM041182]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT25456 NC_015758:c1578049-1577792 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References