Detailed information of TA system TA025454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1387422..1387981
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: MAF_RS06600
• Protein ID: WP_003898789.1
• Coordinates: 1387422..1387715 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: MAF_RS06605
• Protein ID: WP_003406322.1
• Coordinates: 1387712..1387981 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS06575	1383015..1383275	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
MAF_RS06580	1383272..1383703	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
MAF_RS06585	1383726..1385414	-	1689	WP_003910308.1	PE family protein	-
MAF_RS06590	1385594..1386454	+	861	WP_003406306.1	hypothetical protein	-
MAF_RS06595	1386535..1387365	-	831	WP_003910309.1	SDR family oxidoreductase	-
MAF_RS06600	1387422..1387715	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
MAF_RS06605	1387712..1387981	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MAF_RS06610	1388094..1391789	-	3696	WP_031652192.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
MAF_RS06615	1391931..1392719	-	789	WP_013988819.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T25454 WP_003898789.1 NC_015758:c1387715-1387422 [Mycobacterium tuberculosis variant africanum GM041182]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T25454 NC_015758:c1387715-1387422 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT25454 WP_003406322.1 NC_015758:c1387981-1387712 [Mycobacterium tuberculosis variant africanum GM041182]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT25454 NC_015758:c1387981-1387712 [Mycobacterium tuberculosis variant africanum GM041182]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2