Detailed information of TA system TA025453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1383015..1383703
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: MAF_RS06580
• Protein ID: WP_003406304.1
• Coordinates: 1383272..1383703 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: MAF_RS06575
• Protein ID: WP_003406302.1
• Coordinates: 1383015..1383275 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS06555	1378592..1379416	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
MAF_RS06560	1379421..1380602	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
MAF_RS06565	1380679..1381779	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
MAF_RS06570	1381950..1382939	+	990	WP_003910306.1	malate dehydrogenase	-
MAF_RS06575	1383015..1383275	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
MAF_RS06580	1383272..1383703	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS06585	1383726..1385414	-	1689	WP_003910308.1	PE family protein	-
MAF_RS06590	1385594..1386454	+	861	WP_003406306.1	hypothetical protein	-
MAF_RS06595	1386535..1387365	-	831	WP_003910309.1	SDR family oxidoreductase	-
MAF_RS06600	1387422..1387715	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
MAF_RS06605	1387712..1387981	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T25453 WP_003406304.1 NC_015758:1383272-1383703 [Mycobacterium tuberculosis variant africanum GM041182]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T25453 NC_015758:1383272-1383703 [Mycobacterium tuberculosis variant africanum GM041182]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT25453 WP_003406302.1 NC_015758:1383015-1383275 [Mycobacterium tuberculosis variant africanum GM041182]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT25453 NC_015758:1383015-1383275 [Mycobacterium tuberculosis variant africanum GM041182]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6