Detailed information of TA system TA025452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1236801..1237369
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MAF_RS05905
• Protein ID: WP_003405865.1
• Coordinates: 1236995..1237369 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MAF_RS05900
• Protein ID: WP_003405863.1
• Coordinates: 1236801..1236998 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS05880	1232842..1233480	-	639	WP_003898730.1	lipid droplet-associated protein	-
MAF_RS05885	1233570..1234577	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MAF_RS05890	1234594..1235577	-	984	WP_003898731.1	hypothetical protein	-
MAF_RS05895	1235640..1236713	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
MAF_RS05900	1236801..1236998	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAF_RS05905	1236995..1237369	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS05910	1237572..1238270	+	699	WP_003898733.1	hypothetical protein	-
MAF_RS05915	1238388..1238573	+	186	WP_003901093.1	hypothetical protein	-
MAF_RS05920	1238500..1238775	-	276	WP_003405867.1	hypothetical protein	-
MAF_RS05925	1239018..1239341	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
MAF_RS05930	1239356..1240062	-	707	Protein_1157	guanylate cyclase	-
MAF_RS22380	1240084..1240293	+	210	WP_003911400.1	hypothetical protein	-
MAF_RS05940	1240245..1240952	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T25452 WP_003405865.1 NC_015758:1236995-1237369 [Mycobacterium tuberculosis variant africanum GM041182]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T25452 NC_015758:1236995-1237369 [Mycobacterium tuberculosis variant africanum GM041182]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT25452 WP_003405863.1 NC_015758:1236801-1236998 [Mycobacterium tuberculosis variant africanum GM041182]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT25452 NC_015758:1236801-1236998 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07