Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5247018..5247643 Replicon chromosome
Accession NZ_CP102940
Organism Klebsiella pneumoniae strain SCAID PND1-2022 (426)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NW340_RS25505 Protein ID WP_002882817.1
Coordinates 5247018..5247401 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NW340_RS25510 Protein ID WP_004150355.1
Coordinates 5247401..5247643 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NW340_RS25490 (5244384) 5244384..5245286 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NW340_RS25495 (5245283) 5245283..5245918 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NW340_RS25500 (5245915) 5245915..5246844 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NW340_RS25505 (5247018) 5247018..5247401 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NW340_RS25510 (5247401) 5247401..5247643 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NW340_RS25515 (5247848) 5247848..5248765 + 918 WP_032418237.1 alpha/beta hydrolase -
NW340_RS25520 (5248779) 5248779..5249720 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NW340_RS25525 (5249765) 5249765..5250202 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NW340_RS25530 (5250199) 5250199..5251059 - 861 WP_004146232.1 virulence factor BrkB family protein -
NW340_RS25535 (5251053) 5251053..5251652 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254518 WP_002882817.1 NZ_CP102940:c5247401-5247018 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254518 WP_004150355.1 NZ_CP102940:c5247643-5247401 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References