Detailed information of TA system TA025449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1020628..1021541
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: MAF_RS04850
• Protein ID: WP_003404756.1
• Coordinates: 1021041..1021541 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MAF_RS04845
• Protein ID: WP_016719470.1
• Coordinates: 1020628..1021044 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS04825	1016415..1017686	-	1272	WP_003404747.1	PPE family protein	-
MAF_RS04830	1017701..1018000	-	300	WP_003404748.1	PE family protein	-
MAF_RS04835	1018444..1020225	+	1782	WP_003404749.1	BCCT family transporter	-
MAF_RS04840	1020222..1020479	+	258	WP_003404750.1	hypothetical protein	-
MAF_RS04845	1020628..1021044	+	417	WP_016719470.1	DUF1778 domain-containing protein	Antitoxin
MAF_RS04850	1021041..1021541	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
MAF_RS04860	1021854..1023173	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
MAF_RS04865	1023461..1024042	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
MAF_RS04870	1024042..1025694	+	1653	WP_003404761.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (45-136)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T25449 WP_003404756.1 NC_015758:1021041-1021541 [Mycobacterium tuberculosis variant africanum GM041182]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T25449 NC_015758:1021041-1021541 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15278.52 Da
• Isoelectric Point: 10.6899

>AT25449 WP_016719470.1 NC_015758:1020628-1021044 [Mycobacterium tuberculosis variant africanum GM041182]
MAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLTPEQDALIRRAAEAEGTDLTN
FTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 417 bp

>AT25449 NC_015758:1020628-1021044 [Mycobacterium tuberculosis variant africanum GM041182]
ATGGCGCCACGGCCGGTCATCCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAG
CGGCTTGCCAGCGTTGAATTGTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGC
GTCTTGCAGCGCGCCTGACCCCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAAT
TTCACGGTTACAGCGGCGTTGGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTG
GACTGAGTTCCTCGCCGCGCTGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTT
TCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2