Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 577663..578239 Replicon chromosome
Accession NZ_CP102932
Organism Enterobacter sp. Crenshaw

Toxin (Protein)


Gene name shpA Uniprot ID A0A808IJQ6
Locus tag NWV12_RS02675 Protein ID WP_048980720.1
Coordinates 577663..577950 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A808I9W7
Locus tag NWV12_RS02680 Protein ID WP_048980603.1
Coordinates 577937..578239 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWV12_RS02650 (NWV12_02650) 572764..573459 + 696 WP_048980606.1 winged helix-turn-helix domain-containing protein -
NWV12_RS02655 (NWV12_02655) 573609..574079 + 471 WP_033144545.1 MarR family transcriptional regulator -
NWV12_RS02660 (NWV12_02660) 574076..575143 + 1068 WP_101744430.1 HlyD family secretion protein -
NWV12_RS02665 (NWV12_02665) 575148..576224 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
NWV12_RS02670 (NWV12_02670) 576221..577492 - 1272 WP_048980604.1 DUF445 domain-containing protein -
NWV12_RS02675 (NWV12_02675) 577663..577950 + 288 WP_048980720.1 BrnT family toxin Toxin
NWV12_RS02680 (NWV12_02680) 577937..578239 + 303 WP_048980603.1 BrnA antitoxin family protein Antitoxin
NWV12_RS02685 (NWV12_02685) 578270..578908 - 639 WP_048980602.1 LysE family translocator -
NWV12_RS02690 (NWV12_02690) 578956..579699 - 744 WP_048980600.1 AraC family transcriptional regulator -
NWV12_RS02695 (NWV12_02695) 579851..581221 + 1371 WP_048980598.1 NAD-dependent succinate-semialdehyde dehydrogenase -
NWV12_RS02700 (NWV12_02700) 581267..581587 - 321 WP_039262264.1 hypothetical protein -
NWV12_RS02705 (NWV12_02705) 581587..582132 - 546 WP_023310269.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11201.66 Da        Isoelectric Point: 6.7609

>T254488 WP_048980720.1 NZ_CP102932:577663-577950 [Enterobacter sp. Crenshaw]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11451.16 Da        Isoelectric Point: 10.1522

>AT254488 WP_048980603.1 NZ_CP102932:577937-578239 [Enterobacter sp. Crenshaw]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPAMGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IJQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A808I9W7

References