Detailed information of TA system TA254463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2206761..2208586
• Replicon: chromosome
• Accession: NZ_CP102927
• Organism: Vibrio cholerae strain N1252

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: NW313_RS09970
• Protein ID: WP_000392003.1
• Coordinates: 2206761..2208122 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: NW313_RS09975
• Protein ID: WP_000804849.1
• Coordinates: 2208119..2208586 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NW313_RS09955	2202612..2204132	+	1521	WP_000640055.1	aldehyde dehydrogenase family protein	-
NW313_RS09960	2204474..2205154	+	681	Protein_1937	IS5 family transposase	-
NW313_RS09965	2205180..2206157	-	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
NW313_RS09970	2206761..2208122	+	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
NW313_RS09975	2208119..2208586	+	468	WP_000804849.1	helix-turn-helix transcriptional regulator	Antitoxin
NW313_RS09980	2209014..2209592	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
NW313_RS09985	2209596..2212532	+	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
NW313_RS09990	2212602..2213306	+	705	WP_001880605.1	nuclease-related domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfA / acfC / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	2173199..2214149	40950
	-	flank	IS/Tn	-	-	2205180..2205932	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T254463 WP_000392003.1 NZ_CP102927:2206761-2208122 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT254463 WP_000804849.1 NZ_CP102927:2208119-2208586 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8