Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 117476..118128 Replicon plasmid pK18-45_P1
Accession NZ_CP102922
Organism Klebsiella quasipneumoniae strain K18-45

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag NWF33_RS25945 Protein ID WP_017901321.1
Coordinates 117476..117901 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag NWF33_RS25950 Protein ID WP_001261275.1
Coordinates 117898..118128 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWF33_RS25910 (NWF33_25910) 113185..113301 + 117 Protein_121 transposase -
NWF33_RS25915 (NWF33_25915) 113426..113596 - 171 Protein_122 LysR family transcriptional regulator -
NWF33_RS25920 (NWF33_25920) 113607..113834 + 228 Protein_123 IS3 family transposase -
NWF33_RS25925 (NWF33_25925) 113926..115482 - 1557 WP_227639744.1 sensor domain-containing diguanylate cyclase -
NWF33_RS25930 (NWF33_25930) 115669..115842 - 174 Protein_125 nuclease -
NWF33_RS25935 (NWF33_25935) 115895..116431 - 537 Protein_126 integrase core domain-containing protein -
NWF33_RS25940 (NWF33_25940) 116490..117458 - 969 WP_077254762.1 IS5 family transposase -
NWF33_RS25945 (NWF33_25945) 117476..117901 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
NWF33_RS25950 (NWF33_25950) 117898..118128 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
NWF33_RS25955 (NWF33_25955) 118381..120957 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..220751 220751
- flank IS/Tn - - 116490..117458 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T254461 WP_017901321.1 NZ_CP102922:c117901-117476 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT254461 WP_001261275.1 NZ_CP102922:c118128-117898 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References