Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 52314..53224 Replicon plasmid pK18-45_P1
Accession NZ_CP102922
Organism Klebsiella quasipneumoniae strain K18-45

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag NWF33_RS25595 Protein ID WP_004144067.1
Coordinates 52754..53224 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag NWF33_RS25590 Protein ID WP_023287184.1
Coordinates 52314..52757 (+) Length 148 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWF33_RS25570 (NWF33_25570) 47730..48479 + 750 Protein_53 IS5 family transposase -
NWF33_RS25575 (NWF33_25575) 48508..49476 + 969 WP_074424072.1 IS5 family transposase -
NWF33_RS25580 (NWF33_25580) 49672..51018 + 1347 WP_225517490.1 ThiF family adenylyltransferase -
NWF33_RS25585 (NWF33_25585) 51071..52213 + 1143 Protein_56 translesion error-prone DNA polymerase V subunit UmuC -
NWF33_RS25590 (NWF33_25590) 52314..52757 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
NWF33_RS25595 (NWF33_25595) 52754..53224 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
NWF33_RS25600 (NWF33_25600) 53311..54306 + 996 WP_077254794.1 IS5 family transposase -
NWF33_RS25605 (NWF33_25605) 54308..55276 + 969 WP_074423483.1 IS5 family transposase -
NWF33_RS25610 (NWF33_25610) 55615..56826 - 1212 WP_017901247.1 M24 family metallopeptidase -
NWF33_RS25615 (NWF33_25615) 56913..57866 - 954 WP_020481021.1 LysR substrate-binding domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..220751 220751
- inside IScluster/Tn - - 45586..55276 9690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-148)

Antitoxin

(30-89)


Sequences


Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T254459 WP_004144067.1 NZ_CP102922:52754-53224 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp


Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT254459 WP_023287184.1 NZ_CP102922:52314-52757 [Klebsiella quasipneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A384IED8


Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References