25444

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 750373..751006 Replicon chromosome
Accession NC_015758
Organism Mycobacterium africanum GM041182

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag MAF_RS03450 Protein ID WP_003403365.1
Coordinates 750373..750756 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag MAF_RS03455 Protein ID WP_003403368.1
Coordinates 750851..751006 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAF_RS03425 745665..746201 + 537 WP_003403341.1 50S ribosomal protein L10 -
MAF_RS03430 746238..746630 + 393 WP_013988758.1 50S ribosomal protein L7/L12 -
MAF_RS03435 746623..747318 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
MAF_RS03440 747389..748894 + 1506 WP_013988759.1 carotenoid cleavage oxygenase -
MAF_RS03445 748975..749985 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
MAF_RS03450 750373..750756 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
MAF_RS03455 750851..751006 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MAF_RS03460 751082..751798 - 717 WP_013988760.1 CPBP family intramembrane metalloprotease -
MAF_RS03465 752074..752382 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
MAF_RS03470 752369..752614 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
MAF_RS03475 752724..753161 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
MAF_RS03480 753158..753412 - 255 WP_003911263.1 antitoxin VapB7 -
MAF_RS03485 753526..755889 + 2364 WP_003403397.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T25444 WP_003403365.1 NC_015758:c750756-750373 [Mycobacterium tuberculosis variant africanum GM041182]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T25444 NC_015758:c750756-750373 [Mycobacterium tuberculosis variant africanum GM041182]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT25444 WP_003403368.1 NC_015758:c751006-750851 [Mycobacterium tuberculosis variant africanum GM041182]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT25444 NC_015758:c751006-750851 [Mycobacterium tuberculosis variant africanum GM041182]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References