Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 9887..10690 Replicon plasmid pKA1-2
Accession NZ_CP102895
Organism Klebsiella quasipneumoniae strain KA1

Toxin (Protein)


Gene name KacT Uniprot ID G3CAI8
Locus tag NWT75_RS26275 Protein ID WP_000348883.1
Coordinates 9887..10417 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag NWT75_RS26280 Protein ID WP_001275013.1
Coordinates 10421..10690 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT75_RS26240 (NWT75_26240) 5013..5318 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB -
NWT75_RS26245 (NWT75_26245) 5320..5538 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
NWT75_RS26250 (NWT75_26250) 5708..5938 - 231 Protein_7 hypothetical protein -
NWT75_RS26255 (NWT75_26255) 6250..8871 + 2622 WP_000283688.1 histidinol-phosphatase -
NWT75_RS26260 (NWT75_26260) 9070..9300 + 231 WP_001261274.1 type II toxin-antitoxin system VapB family antitoxin -
NWT75_RS26265 (NWT75_26265) 9297..9613 + 317 Protein_10 type II toxin-antitoxin system VapC family toxin -
NWT75_RS26270 (NWT75_26270) 9669..9886 - 218 Protein_11 transposase -
NWT75_RS26275 (NWT75_26275) 9887..10417 - 531 WP_000348883.1 GNAT family N-acetyltransferase Toxin
NWT75_RS26280 (NWT75_26280) 10421..10690 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
NWT75_RS26285 (NWT75_26285) 11572..12723 + 1152 WP_001254932.1 IS30-like element IS30 family transposase -
NWT75_RS26290 (NWT75_26290) 12733..13638 + 906 Protein_15 IS3 family transposase -
NWT75_RS26295 (NWT75_26295) 14543..14719 - 177 Protein_16 ferric citrate ABC transporter ATP-binding protein FecE -
NWT75_RS26300 (NWT75_26300) 14825..15661 - 837 WP_097474928.1 APH(6)-I family aminoglycoside O-phosphotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / tet(A) / sul1 / qnrB2 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / floR / sul2 / aph(4)-Ia / aac(3)-IVa - 1..85913 85913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20321.20 Da        Isoelectric Point: 6.4889

>T254429 WP_000348883.1 NZ_CP102895:c10417-9887 [Klebsiella quasipneumoniae]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT254429 WP_001275013.1 NZ_CP102895:c10690-10421 [Klebsiella quasipneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAI8

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References