Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5195021..5195646 Replicon chromosome
Accession NZ_CP102889
Organism Klebsiella pneumoniae strain KW2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NWT79_RS25230 Protein ID WP_002882817.1
Coordinates 5195021..5195404 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NWT79_RS25235 Protein ID WP_004150355.1
Coordinates 5195404..5195646 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT79_RS25215 (NWT79_25215) 5192387..5193289 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NWT79_RS25220 (NWT79_25220) 5193286..5193921 + 636 WP_040189416.1 formate dehydrogenase cytochrome b556 subunit -
NWT79_RS25225 (NWT79_25225) 5193918..5194847 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NWT79_RS25230 (NWT79_25230) 5195021..5195404 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NWT79_RS25235 (NWT79_25235) 5195404..5195646 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NWT79_RS25240 (NWT79_25240) 5195851..5196768 + 918 WP_004181612.1 alpha/beta hydrolase -
NWT79_RS25245 (NWT79_25245) 5196782..5197723 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NWT79_RS25250 (NWT79_25250) 5197768..5198205 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NWT79_RS25255 (NWT79_25255) 5198202..5199062 - 861 WP_004146232.1 virulence factor BrkB family protein -
NWT79_RS25260 (NWT79_25260) 5199056..5199655 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254402 WP_002882817.1 NZ_CP102889:c5195404-5195021 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254402 WP_004150355.1 NZ_CP102889:c5195646-5195404 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References