Detailed information of TA system TA025440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 700105..700748
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: MAF_RS03175
• Protein ID: WP_003403187.1
• Coordinates: 700347..700748 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: MAF_RS03170
• Protein ID: WP_003403184.1
• Coordinates: 700105..700350 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS03135	695387..695917	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MAF_RS03140	695901..696596	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MAF_RS21855	696858..697028	+	171	WP_156509370.1	hypothetical protein	-
MAF_RS03150	697100..698050	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
MAF_RS03155	698291..698875	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MAF_RS03160	698877..699587	+	711	Protein_625	transposase	-
MAF_RS03165	699590..700060	+	471	WP_003898523.1	hypothetical protein	-
MAF_RS03170	700105..700350	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MAF_RS03175	700347..700748	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS21860	701136..701303	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
MAF_RS21865	701336..701533	-	198	WP_003403191.1	hypothetical protein	-
MAF_RS03190	701613..702770	-	1158	WP_003403193.1	hypothetical protein	-
MAF_RS03195	702822..703043	-	222	WP_031666828.1	DUF4926 domain-containing protein	-
MAF_RS03200	703185..703790	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T25440 WP_003403187.1 NC_015758:700347-700748 [Mycobacterium tuberculosis variant africanum GM041182]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T25440 NC_015758:700347-700748 [Mycobacterium tuberculosis variant africanum GM041182]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCTGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT25440 WP_003403184.1 NC_015758:700105-700350 [Mycobacterium tuberculosis variant africanum GM041182]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT25440 NC_015758:700105-700350 [Mycobacterium tuberculosis variant africanum GM041182]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91