Detailed information of TA system TA025439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 694017..694663
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: MAF_RS03120
• Protein ID: WP_003403137.1
• Coordinates: 694017..694430 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: MAF_RS03125
• Protein ID: WP_003403139.1
• Coordinates: 694427..694663 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS03100	690100..691650	+	1551	WP_003403119.1	MCE family protein	-
MAF_RS03105	691702..692094	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
MAF_RS03110	692091..692348	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
MAF_RS03115	692531..693766	-	1236	WP_003403128.1	ATP-binding protein	-
MAF_RS03120	694017..694430	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
MAF_RS03125	694427..694663	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MAF_RS03130	694767..695273	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MAF_RS03135	695387..695917	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MAF_RS03140	695901..696596	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MAF_RS21855	696858..697028	+	171	WP_156509370.1	hypothetical protein	-
MAF_RS03150	697100..698050	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
MAF_RS03155	698291..698875	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
MAF_RS03160	698877..699587	+	711	Protein_625	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T25439 WP_003403137.1 NC_015758:c694430-694017 [Mycobacterium tuberculosis variant africanum GM041182]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T25439 NC_015758:c694430-694017 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT25439 WP_003403139.1 NC_015758:c694663-694427 [Mycobacterium tuberculosis variant africanum GM041182]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT25439 NC_015758:c694663-694427 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89