Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 30413..30938 Replicon plasmid pKH2-3-mcr8.1
Accession NZ_CP102886
Organism Klebsiella pneumoniae strain KH2

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag M2890_RS28010 Protein ID WP_013023785.1
Coordinates 30633..30938 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag M2890_RS28005 Protein ID WP_001568025.1
Coordinates 30413..30631 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2890_RS27985 (M2890_27985) 25660..26628 + 969 WP_075211999.1 IS5 family transposase -
M2890_RS27990 (M2890_27990) 27100..27891 - 792 WP_221928726.1 ribbon-helix-helix domain-containing protein -
M2890_RS27995 (M2890_27995) 28074..29102 - 1029 WP_032445668.1 Abi family protein -
M2890_RS28000 (M2890_28000) 29263..29844 - 582 WP_072310991.1 hypothetical protein -
M2890_RS28005 (M2890_28005) 30413..30631 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M2890_RS28010 (M2890_28010) 30633..30938 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
M2890_RS28015 (M2890_28015) 31107..31502 + 396 WP_017899885.1 hypothetical protein -
M2890_RS28020 (M2890_28020) 31529..31852 + 324 WP_004197641.1 hypothetical protein -
M2890_RS28025 (M2890_28025) 31849..32865 + 1017 WP_118842534.1 hypothetical protein -
M2890_RS28030 (M2890_28030) 33063..33848 + 786 WP_046664219.1 site-specific integrase -
M2890_RS28035 (M2890_28035) 34297..35052 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-8 - 1..100448 100448
- flank IS/Tn mcr-8 - 19604..26628 7024


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T254389 WP_013023785.1 NZ_CP102886:30633-30938 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT254389 WP_001568025.1 NZ_CP102886:30413-30631 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References