Detailed information of TA system TA254380

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4665955..4666681
• Replicon: chromosome
• Accession: NZ_CP102883
• Organism: Klebsiella pneumoniae strain KH2

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: M2890_RS23075
• Protein ID: WP_032437356.1
• Coordinates: 4665955..4666296 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: M2890_RS23080
• Protein ID: WP_032437354.1
• Coordinates: 4666331..4666681 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2890_RS23045 (4660962)	4660962..4662233	+	1272	Protein_4517	conjugative transfer system coupling protein TraD	-
M2890_RS23050 (4662196)	4662196..4662462	+	267	WP_004178415.1	hypothetical protein	-
M2890_RS23055 (4662462)	4662462..4663134	+	673	Protein_4519	DUF4400 domain-containing protein	-
M2890_RS23060 (4663145)	4663145..4664029	+	885	WP_004192285.1	RES domain-containing protein	-
M2890_RS23065 (4664228)	4664228..4664416	-	189	Protein_4521	transposase	-
M2890_RS23070 (4664433)	4664433..4665544	-	1112	Protein_4522	IS3 family transposase	-
M2890_RS23075 (4665955)	4665955..4666296	-	342	WP_032437356.1	TA system toxin CbtA family protein	Toxin
M2890_RS23080 (4666331)	4666331..4666681	-	351	WP_032437354.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
M2890_RS23085 (4666702)	4666702..4666923	-	222	WP_063931680.1	DUF987 domain-containing protein	-
M2890_RS23090 (4666939)	4666939..4667412	-	474	WP_032437352.1	DNA repair protein RadC	-
M2890_RS23095 (4667483)	4667483..4668304	-	822	WP_187982516.1	DUF932 domain-containing protein	-
M2890_RS23100 (4668400)	4668400..4669077	-	678	WP_032437348.1	hypothetical protein	-
M2890_RS23105 (4669363)	4669363..4670256	-	894	WP_032437346.1	50S ribosome-binding GTPase	-
M2890_RS23110 (4670354)	4670354..4671505	+	1152	WP_129075233.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4656307..4695135	38828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12991.04 Da
• Isoelectric Point: 5.0978

>T254380 WP_032437356.1 NZ_CP102883:c4666296-4665955 [Klebsiella pneumoniae]
MNTLPVINQRAIQLCPSPVTIWQTLLTRLLEQHYGLALNDTPFGDESVIQEHIEAGITLVDAVNFLVEKYELVRIDRWAF
GWLEPSPYLRAEDILRMRRDMGLLRGCNHTAAM

Nucleotide

• Length: 342 bp

>T254380 NZ_CP102883:c4666296-4665955 [Klebsiella pneumoniae]
ATGAATACTTTACCTGTAATAAACCAGCGGGCTATTCAGTTATGCCCATCGCCTGTAACTATCTGGCAAACGTTGCTAAC
TCGTTTACTGGAACAACACTACGGGCTGGCGCTGAACGACACACCGTTCGGTGATGAAAGCGTGATTCAGGAACATATCG
AGGCCGGTATTACACTCGTTGATGCCGTTAACTTCTTGGTGGAAAAGTACGAGCTGGTTCGTATCGACCGCTGGGCGTTT
GGCTGGTTGGAACCATCTCCATACTTGCGAGCTGAAGACATTCTGCGAATGCGTCGGGACATGGGGCTGTTGCGAGGCTG
CAACCACACTGCAGCAATGTGA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12859.60 Da
• Isoelectric Point: 6.3108

>AT254380 WP_032437354.1 NZ_CP102883:c4666681-4666331 [Klebsiella pneumoniae]
MSNKTQTVNGDTAEPRWGLSCNVIPCFGARLVQEGNRLHYLADRASITGQFNEADLIHPDQAFPVLLKQAELMLTSGELN
PRHQHCVTFCEKGLTCEADTLGSCGHVYIVIYPTQR

Nucleotide

• Length: 351 bp

>AT254380 NZ_CP102883:c4666681-4666331 [Klebsiella pneumoniae]
ATGTCGAATAAGACACAGACAGTGAATGGTGATACTGCCGAGCCCAGGTGGGGCTTGAGTTGTAACGTCATCCCTTGTTT
TGGTGCACGTCTGGTCCAGGAAGGTAACCGCCTACATTATCTGGCTGACCGCGCCAGCATAACAGGTCAGTTCAATGAAG
CGGATTTAATCCATCCTGACCAGGCATTCCCGGTCCTGTTGAAGCAGGCAGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACGTTCTGTGAAAAGGGGTTGACCTGCGAAGCTGATACGCTGGGTTCCTGTGGCCACGT
CTATATTGTCATTTACCCTACTCAACGCTAA