Detailed information of TA system TA025438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 691702..692348
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: MAF_RS03105
• Protein ID: WP_003403122.1
• Coordinates: 691702..692094 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: MAF_RS03110
• Protein ID: WP_003403125.1
• Coordinates: 692091..692348 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS03090	687364..688890	+	1527	WP_003403112.1	virulence factor Mce family protein	-
MAF_RS03095	688953..690095	+	1143	WP_003911253.1	virulence factor Mce family protein	-
MAF_RS03100	690100..691650	+	1551	WP_003403119.1	MCE family protein	-
MAF_RS03105	691702..692094	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
MAF_RS03110	692091..692348	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
MAF_RS03115	692531..693766	-	1236	WP_003403128.1	ATP-binding protein	-
MAF_RS03120	694017..694430	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
MAF_RS03125	694427..694663	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MAF_RS03130	694767..695273	-	507	WP_003900973.1	two component system sensor kinase HK1	-
MAF_RS03135	695387..695917	-	531	WP_003917320.1	two component system sensor kinase HK2	-
MAF_RS03140	695901..696596	-	696	WP_003918099.1	two-component system response regulator TcrA	-
MAF_RS21855	696858..697028	+	171	WP_156509370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T25438 WP_003403122.1 NC_015758:c692094-691702 [Mycobacterium tuberculosis variant africanum GM041182]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T25438 NC_015758:c692094-691702 [Mycobacterium tuberculosis variant africanum GM041182]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT25438 WP_003403125.1 NC_015758:c692348-692091 [Mycobacterium tuberculosis variant africanum GM041182]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT25438 NC_015758:c692348-692091 [Mycobacterium tuberculosis variant africanum GM041182]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86