Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4627140..4627950 Replicon chromosome
Accession NZ_CP102877
Organism Klebsiella pneumoniae strain KH1

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag NWT87_RS22855 Protein ID WP_023279404.1
Coordinates 4627140..4627673 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NWT87_RS22860 Protein ID WP_002887278.1
Coordinates 4627684..4627950 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT87_RS22850 (4625971) 4625971..4627092 + 1122 WP_002887282.1 cupin domain-containing protein -
NWT87_RS22855 (4627140) 4627140..4627673 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
NWT87_RS22860 (4627684) 4627684..4627950 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NWT87_RS22865 (4628053) 4628053..4629486 - 1434 WP_258323903.1 Cu(+)/Ag(+) sensor histidine kinase -
NWT87_RS22870 (4629476) 4629476..4630159 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NWT87_RS22875 (4630331) 4630331..4631716 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
NWT87_RS22880 (4631734) 4631734..4632078 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T254358 WP_023279404.1 NZ_CP102877:c4627673-4627140 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT254358 WP_002887278.1 NZ_CP102877:c4627950-4627684 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References