Detailed information of TA system TA025435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 360591..361234
• Replicon: chromosome
• Accession: NC_015758
• Organism: Mycobacterium africanum GM041182

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MAF_RS01595
• Protein ID: WP_013988730.1
• Coordinates: 360809..361234 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: MAF_RS01590
• Protein ID: WP_003401563.1
• Coordinates: 360591..360812 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAF_RS01565	355665..356468	-	804	WP_003401540.1	hypothetical protein	-
MAF_RS01570	356478..357875	-	1398	WP_013988728.1	sulfatase	-
MAF_RS01575	358054..359874	+	1821	WP_003401546.1	PE family protein	-
MAF_RS01580	360017..360244	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
MAF_RS01585	360241..360543	+	303	WP_003401560.1	toxin-antitoxin system toxin	-
MAF_RS01590	360591..360812	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
MAF_RS01595	360809..361234	+	426	WP_013988730.1	PIN domain nuclease	Toxin
MAF_RS01600	361370..362002	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
MAF_RS01605	361999..362907	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15694.86 Da
• Isoelectric Point: 5.5587

>T25435 WP_013988730.1 NC_015758:360809-361234 [Mycobacterium tuberculosis variant africanum GM041182]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHHGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T25435 NC_015758:360809-361234 [Mycobacterium tuberculosis variant africanum GM041182]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCATGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT25435 WP_003401563.1 NC_015758:360591-360812 [Mycobacterium tuberculosis variant africanum GM041182]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT25435 NC_015758:360591-360812 [Mycobacterium tuberculosis variant africanum GM041182]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48