Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84860..86483 Replicon plasmid pKA2-2
Accession NZ_CP102871
Organism Klebsiella pneumoniae strain KA2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NWT85_RS27575 Protein ID WP_000088046.1
Coordinates 85158..86483 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NWT85_RS27570 Protein ID WP_024136327.1
Coordinates 84860..85153 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT85_RS27540 (NWT85_27540) 80732..81973 + 1242 WP_001053338.1 VWA domain-containing protein -
NWT85_RS27545 (NWT85_27545) 82064..82195 + 132 WP_000374059.1 hypothetical protein -
NWT85_RS27550 (NWT85_27550) 82536..82817 - 282 WP_000077926.1 hypothetical protein -
NWT85_RS27555 (NWT85_27555) 82867..83058 - 192 WP_000398480.1 hypothetical protein -
NWT85_RS27560 (NWT85_27560) 83150..83491 - 342 WP_001151575.1 hypothetical protein -
NWT85_RS27565 (NWT85_27565) 83864..84256 + 393 WP_000341066.1 hypothetical protein -
NWT85_RS27570 (NWT85_27570) 84860..85153 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NWT85_RS27575 (NWT85_27575) 85158..86483 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NWT85_RS27580 (NWT85_27580) 86544..86750 + 207 WP_000134171.1 hypothetical protein -
NWT85_RS27585 (NWT85_27585) 86852..87262 + 411 WP_000985911.1 hypothetical protein -
NWT85_RS27590 (NWT85_27590) 87275..88090 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NWT85_RS27595 (NWT85_27595) 88344..88769 + 426 WP_001043843.1 hypothetical protein -
NWT85_RS27600 (NWT85_27600) 89223..89459 + 237 Protein_87 type I restriction-modification system subunit M N-terminal domain-containing protein -
NWT85_RS27605 (NWT85_27605) 89514..89813 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..134407 134407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T254344 WP_000088046.1 NZ_CP102871:85158-86483 [Klebsiella pneumoniae]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT254344 WP_024136327.1 NZ_CP102871:84860-85153 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References