Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5208183..5208808 Replicon chromosome
Accession NZ_CP102869
Organism Klebsiella pneumoniae strain KA2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NWT85_RS25395 Protein ID WP_002882817.1
Coordinates 5208183..5208566 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NWT85_RS25400 Protein ID WP_004150355.1
Coordinates 5208566..5208808 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT85_RS25380 (NWT85_25380) 5205549..5206451 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NWT85_RS25385 (NWT85_25385) 5206448..5207083 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NWT85_RS25390 (NWT85_25390) 5207080..5208009 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NWT85_RS25395 (NWT85_25395) 5208183..5208566 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NWT85_RS25400 (NWT85_25400) 5208566..5208808 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NWT85_RS25405 (NWT85_25405) 5209013..5209930 + 918 WP_032422045.1 alpha/beta hydrolase -
NWT85_RS25410 (NWT85_25410) 5209944..5210885 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NWT85_RS25415 (NWT85_25415) 5210930..5211367 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NWT85_RS25420 (NWT85_25420) 5211364..5212224 - 861 WP_004146232.1 virulence factor BrkB family protein -
NWT85_RS25425 (NWT85_25425) 5212218..5212817 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254340 WP_002882817.1 NZ_CP102869:c5208566-5208183 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254340 WP_004150355.1 NZ_CP102869:c5208808-5208566 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References