Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4607019..4607829 Replicon chromosome
Accession NZ_CP102869
Organism Klebsiella pneumoniae strain KA2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NWT85_RS22650 Protein ID WP_004178461.1
Coordinates 4607019..4607552 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NWT85_RS22655 Protein ID WP_002887278.1
Coordinates 4607563..4607829 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWT85_RS22645 (NWT85_22645) 4605850..4606971 + 1122 WP_258334886.1 cupin domain-containing protein -
NWT85_RS22650 (NWT85_22650) 4607019..4607552 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NWT85_RS22655 (NWT85_22655) 4607563..4607829 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NWT85_RS22660 (NWT85_22660) 4607932..4609365 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
NWT85_RS22665 (NWT85_22665) 4609355..4610038 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NWT85_RS22670 (NWT85_22670) 4610211..4611596 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
NWT85_RS22675 (NWT85_22675) 4611614..4611958 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T254338 WP_004178461.1 NZ_CP102869:c4607552-4607019 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT254338 WP_002887278.1 NZ_CP102869:c4607829-4607563 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References