254327

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1274644..1275561 Replicon chromosome
Accession NZ_CP102866
Organism Bacillus velezensis strain YS-AT-DS1

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag NWE25_RS06355 Protein ID WP_007407256.1
Coordinates 1274815..1275561 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag NWE25_RS06350 Protein ID WP_003154807.1
Coordinates 1274644..1274814 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWE25_RS06300 (NWE25_06300) 1269796..1270308 + 513 WP_032874577.1 sigma-70 family RNA polymerase sigma factor -
NWE25_RS06305 (NWE25_06305) 1270421..1270747 + 327 WP_228842184.1 hypothetical protein -
NWE25_RS06310 (NWE25_06310) 1270857..1271498 + 642 WP_228842183.1 hypothetical protein -
NWE25_RS06315 (NWE25_06315) 1271511..1271882 + 372 WP_038457736.1 XkdW family protein -
NWE25_RS06320 (NWE25_06320) 1271887..1272084 + 198 WP_007610833.1 XkdX family protein -
NWE25_RS06325 (NWE25_06325) 1272141..1272902 + 762 WP_038457737.1 hypothetical protein -
NWE25_RS06330 (NWE25_06330) 1272955..1273218 + 264 WP_003154815.1 hemolysin XhlA family protein -
NWE25_RS06335 (NWE25_06335) 1273232..1273495 + 264 WP_003154813.1 phage holin -
NWE25_RS06340 (NWE25_06340) 1273509..1274387 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
NWE25_RS06345 (NWE25_06345) 1274422..1274547 - 126 WP_003154809.1 hypothetical protein -
NWE25_RS06350 (NWE25_06350) 1274644..1274814 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
NWE25_RS06355 (NWE25_06355) 1274815..1275561 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
NWE25_RS06360 (NWE25_06360) 1275666..1276664 - 999 WP_032874611.1 inorganic phosphate transporter -
NWE25_RS06365 (NWE25_06365) 1276677..1277294 - 618 WP_032874614.1 DUF47 domain-containing protein -
NWE25_RS06370 (NWE25_06370) 1277579..1278895 - 1317 WP_038457739.1 amino acid permease -
NWE25_RS06375 (NWE25_06375) 1279218..1280168 + 951 WP_007610844.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T254327 WP_007407256.1 NZ_CP102866:c1275561-1274815 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT254327 WP_003154807.1 NZ_CP102866:c1274814-1274644 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References