Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 482201..482838 Replicon chromosome
Accession NZ_CP102866
Organism Bacillus velezensis strain YS-AT-DS1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NWE25_RS02475 Protein ID WP_003156187.1
Coordinates 482488..482838 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NWE25_RS02470 Protein ID WP_003156188.1
Coordinates 482201..482482 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWE25_RS02450 (NWE25_02450) 478566..479165 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
NWE25_RS02455 (NWE25_02455) 479258..479623 + 366 WP_007609580.1 holo-ACP synthase -
NWE25_RS02460 (NWE25_02460) 479788..480795 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
NWE25_RS02465 (NWE25_02465) 480912..482081 + 1170 WP_038457028.1 alanine racemase -
NWE25_RS02470 (NWE25_02470) 482201..482482 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NWE25_RS02475 (NWE25_02475) 482488..482838 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NWE25_RS02480 (NWE25_02480) 482956..483777 + 822 WP_003156182.1 STAS domain-containing protein -
NWE25_RS02485 (NWE25_02485) 483782..484147 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
NWE25_RS02490 (NWE25_02490) 484150..484551 + 402 WP_003156178.1 anti-sigma regulatory factor -
NWE25_RS02495 (NWE25_02495) 484563..485570 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
NWE25_RS02500 (NWE25_02500) 485634..485963 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NWE25_RS02505 (NWE25_02505) 485960..486442 + 483 WP_007609591.1 anti-sigma B factor RsbW -
NWE25_RS02510 (NWE25_02510) 486408..487196 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NWE25_RS02515 (NWE25_02515) 487196..487798 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T254326 WP_003156187.1 NZ_CP102866:482488-482838 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT254326 WP_003156188.1 NZ_CP102866:482201-482482 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References