Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 373877..374528 Replicon chromosome
Accession NZ_CP102859
Organism Lacticaseibacillus paracasei strain DM001

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag NJN40_RS01810 Protein ID WP_003567661.1
Coordinates 374145..374528 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag NJN40_RS01805 Protein ID WP_003567665.1
Coordinates 373877..374125 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NJN40_RS01780 (NJN40_01780) 368946..370334 + 1389 WP_253613061.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
NJN40_RS01785 (NJN40_01785) 370365..370508 + 144 WP_003591975.1 hypothetical protein -
NJN40_RS01790 (NJN40_01790) 370634..372142 + 1509 WP_003567670.1 DEAD/DEAH box helicase -
NJN40_RS01795 (NJN40_01795) 372312..372686 + 375 WP_253613065.1 holo-ACP synthase -
NJN40_RS01800 (NJN40_01800) 372673..373809 + 1137 WP_253613067.1 alanine racemase -
NJN40_RS01805 (NJN40_01805) 373877..374125 + 249 WP_003567665.1 antitoxin Antitoxin
NJN40_RS01810 (NJN40_01810) 374145..374528 + 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NJN40_RS01815 (NJN40_01815) 374712..375233 + 522 WP_128538209.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T254324 WP_003567661.1 NZ_CP102859:374145-374528 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT254324 WP_003567665.1 NZ_CP102859:373877-374125 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References