Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84084..85707 Replicon plasmid p573-1
Accession NZ_CP102854
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NWH85_RS23225 Protein ID WP_000088046.1
Coordinates 84382..85707 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NWH85_RS23220 Protein ID WP_024136327.1
Coordinates 84084..84377 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS23190 (NWH85_23235) 79956..81197 + 1242 WP_001053338.1 VWA domain-containing protein -
NWH85_RS23195 (NWH85_23240) 81288..81419 + 132 WP_000374059.1 hypothetical protein -
NWH85_RS23200 (NWH85_23245) 81760..82041 - 282 WP_000077926.1 hypothetical protein -
NWH85_RS23205 (NWH85_23250) 82091..82282 - 192 WP_000398480.1 hypothetical protein -
NWH85_RS23210 (NWH85_23255) 82374..82715 - 342 WP_001151575.1 hypothetical protein -
NWH85_RS23215 (NWH85_23260) 83088..83480 + 393 WP_000341066.1 hypothetical protein -
NWH85_RS23220 (NWH85_23265) 84084..84377 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NWH85_RS23225 (NWH85_23270) 84382..85707 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NWH85_RS23230 (NWH85_23275) 85768..85974 + 207 WP_000134171.1 hypothetical protein -
NWH85_RS23235 (NWH85_23280) 86076..86486 + 411 WP_000985911.1 hypothetical protein -
NWH85_RS23240 (NWH85_23285) 86499..87314 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NWH85_RS23245 (NWH85_23290) 87568..87993 + 426 WP_001043843.1 hypothetical protein -
NWH85_RS23250 (NWH85_23295) 88542..88850 + 309 WP_001224687.1 hypothetical protein -
NWH85_RS23255 (NWH85_23300) 88866..89723 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
NWH85_RS23260 (NWH85_23305) 89785..89988 + 204 WP_001194555.1 hypothetical protein -
NWH85_RS23265 (NWH85_23310) 90330..90686 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / dfrA12 - 1..233164 233164


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T254307 WP_000088046.1 NZ_CP102854:84382-85707 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT254307 WP_024136327.1 NZ_CP102854:84084-84377 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References