254302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3480285..3481122 Replicon chromosome
Accession NZ_CP102853
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NWH85_RS17095 Protein ID WP_000227784.1
Coordinates 3480580..3481122 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NWH85_RS17090 Protein ID WP_001297137.1
Coordinates 3480285..3480596 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS17065 (3475305) 3475305..3476252 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
NWH85_RS17070 (3476274) 3476274..3478265 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NWH85_RS17075 (3478255) 3478255..3478869 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NWH85_RS17080 (3478869) 3478869..3479198 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NWH85_RS17085 (3479210) 3479210..3480100 + 891 WP_000971336.1 heme o synthase -
NWH85_RS17090 (3480285) 3480285..3480596 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NWH85_RS17095 (3480580) 3480580..3481122 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NWH85_RS17100 (3481178) 3481178..3482113 - 936 WP_001297127.1 tetratricopeptide repeat protein -
NWH85_RS17105 (3482521) 3482521..3483885 + 1365 WP_001000978.1 MFS transporter -
NWH85_RS17110 (3484013) 3484013..3484504 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NWH85_RS17115 (3484672) 3484672..3485583 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T254302 WP_000227784.1 NZ_CP102853:3480580-3481122 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT254302 WP_001297137.1 NZ_CP102853:3480285-3480596 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References