Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1000062..1000645 Replicon chromosome
Accession NZ_CP102853
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag NWH85_RS04835 Protein ID WP_000254745.1
Coordinates 1000310..1000645 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NWH85_RS04830 Protein ID WP_000581937.1
Coordinates 1000062..1000310 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS04820 (996401) 996401..997702 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
NWH85_RS04825 (997750) 997750..999984 + 2235 WP_000226815.1 GTP pyrophosphokinase -
NWH85_RS04830 (1000062) 1000062..1000310 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NWH85_RS04835 (1000310) 1000310..1000645 + 336 WP_000254745.1 endoribonuclease MazF Toxin
NWH85_RS04840 (1000716) 1000716..1001507 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NWH85_RS04845 (1001735) 1001735..1003372 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NWH85_RS04850 (1003460) 1003460..1004758 + 1299 WP_000036723.1 phosphopyruvate hydratase -
NWH85_RS04855 (1004814) 1004814..1005176 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T254292 WP_000254745.1 NZ_CP102853:1000310-1000645 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT254292 WP_000581937.1 NZ_CP102853:1000062-1000310 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References