Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 287766..288566 Replicon chromosome
Accession NZ_CP102853
Organism Escherichia coli strain 573

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NWH85_RS01320 Protein ID WP_000342449.1
Coordinates 288039..288566 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NWH85_RS01315 Protein ID WP_001277108.1
Coordinates 287766..288032 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NWH85_RS01295 (283424) 283424..284092 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NWH85_RS01300 (284085) 284085..285143 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NWH85_RS01305 (285388) 285388..286242 + 855 WP_063100092.1 RNA polymerase sigma factor RpoH -
NWH85_RS01310 (286513) 286513..287616 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NWH85_RS01315 (287766) 287766..288032 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NWH85_RS01320 (288039) 288039..288566 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NWH85_RS01325 (288563) 288563..288946 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
NWH85_RS01330 (289370) 289370..290479 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NWH85_RS01335 (290527) 290527..291453 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NWH85_RS01340 (291450) 291450..292727 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
NWH85_RS01345 (292724) 292724..293491 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T254289 WP_000342449.1 NZ_CP102853:288039-288566 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT254289 WP_001277108.1 NZ_CP102853:287766-288032 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References