Detailed information of TA system TA025428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2127720..2128341
• Replicon: chromosome
• Accession: NC_015738
• Organism: Eggerthella sp. YY7918

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F7UV54
• Locus tag: EGYY_RS08940
• Protein ID: WP_013980321.1
• Coordinates: 2128009..2128341 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F7UV53
• Locus tag: EGYY_RS08935
• Protein ID: WP_013980320.1
• Coordinates: 2127720..2128025 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGYY_RS08925	2125470..2126453	+	984	WP_013980318.1	P1 family peptidase	-
EGYY_RS08930	2126465..2127736	+	1272	WP_013980319.1	chloride channel protein	-
EGYY_RS08935	2127720..2128025	-	306	WP_013980320.1	putative addiction module antidote protein	Antitoxin
EGYY_RS08940	2128009..2128341	-	333	WP_013980321.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGYY_RS08945	2128675..2129016	-	342	WP_151197565.1	ribosome silencing factor	-
EGYY_RS08950	2129129..2129812	-	684	WP_013980323.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EGYY_RS08955	2129809..2130477	-	669	WP_013980324.1	nicotinate-nucleotide adenylyltransferase	-
EGYY_RS08960	2130477..2131754	-	1278	WP_041690720.1	glutamate-5-semialdehyde dehydrogenase	-
EGYY_RS08965	2131764..2132912	-	1149	WP_013980326.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (7-101)

Antitoxin

• Uncharacterized protein HI1420 (21-99)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12430.42 Da
• Isoelectric Point: 10.8304

>T25428 WP_013980321.1 NC_015738:c2128341-2128009 [Eggerthella sp. YY7918]
MGQETWTVKKTRTFERWLRRLKDRKARGRISARVFRLEEGLLGDVRSVGEGVFELRIDYGPGYRLYGTIMGAVVIVLLCA
GDKGSQKRDIARAKRMAARLKAGDGDEESL

Nucleotide

• Length: 333 bp

>T25428 NC_015738:c2128341-2128009 [Eggerthella sp. YY7918]
ATGGGTCAGGAAACGTGGACAGTCAAGAAAACTCGGACGTTTGAGCGTTGGCTGCGCAGGCTGAAAGATCGGAAGGCTCG
AGGTCGTATATCAGCCCGGGTATTTCGCTTGGAAGAAGGGTTGCTCGGTGATGTGCGGTCAGTAGGCGAGGGTGTGTTTG
AGCTGCGGATCGATTATGGTCCAGGGTATCGGCTATACGGCACCATAATGGGAGCAGTAGTTATTGTGCTCTTATGCGCA
GGAGATAAAGGTTCTCAGAAAAGGGATATTGCTCGCGCGAAAAGAATGGCTGCAAGGTTGAAGGCAGGAGATGGTGATGA
AGAATCCCTTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11003.47 Da
• Isoelectric Point: 4.8228

>AT25428 WP_013980320.1 NC_015738:c2128025-2127720 [Eggerthella sp. YY7918]
MKNPFKKSIEISEFHAYDYLDSNETIAAFLSAALEENDPEFFQKALGEVARARGMAAVAQKAGVGRESLYKSLSAEGNPR
FATIMDVLDALGFKLTVTPNA

Nucleotide

• Length: 306 bp

>AT25428 NC_015738:c2128025-2127720 [Eggerthella sp. YY7918]
ATGAAGAATCCCTTTAAAAAATCGATAGAGATTTCCGAATTTCATGCATACGATTATCTTGATTCCAACGAGACTATCGC
CGCGTTTCTTTCTGCTGCACTTGAGGAAAACGACCCCGAATTTTTCCAAAAAGCACTCGGTGAGGTTGCTCGTGCGCGGG
GTATGGCTGCGGTGGCGCAGAAAGCAGGGGTGGGGCGCGAGAGCCTGTATAAGTCGCTTTCAGCTGAAGGCAATCCGCGG
TTTGCTACGATCATGGACGTGCTCGATGCTTTGGGTTTCAAGCTGACGGTCACGCCGAATGCGTGA

Structures

Toxin

• AlphaFold DB: F7UV54

Antitoxin

• AlphaFold DB: F7UV53