Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 35164..35741 Replicon plasmid pKP4863-1
Accession NZ_CP102842
Organism Klebsiella pneumoniae strain KP4863

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag NV397_RS25700 Protein ID WP_059065587.1
Coordinates 35409..35741 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag NV397_RS25695 Protein ID WP_032454911.1
Coordinates 35164..35409 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV397_RS25680 (NV397_25680) 32198..33418 - 1221 WP_094313437.1 HlyD family efflux transporter periplasmic adaptor subunit -
NV397_RS25685 (NV397_25685) 33951..34526 - 576 WP_094313438.1 hypothetical protein -
NV397_RS25690 (NV397_25690) 34661..34789 - 129 WP_094308947.1 IS1 family transposase -
NV397_RS25695 (NV397_25695) 35164..35409 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
NV397_RS25700 (NV397_25700) 35409..35741 + 333 WP_059065587.1 endoribonuclease MazF Toxin
NV397_RS25705 (NV397_25705) 35806..35898 + 93 Protein_40 IS3 family transposase -
NV397_RS25710 (NV397_25710) 36091..36351 - 261 Protein_41 hypothetical protein -
NV397_RS25715 (NV397_25715) 36305..36607 - 303 Protein_42 antirestriction protein -
NV397_RS25720 (NV397_25720) 36893..37123 - 231 WP_004118481.1 hypothetical protein -
NV397_RS25725 (NV397_25725) 37355..38764 - 1410 Protein_44 group II intron reverse transcriptase/maturase -
NV397_RS25730 (NV397_25730) 38807..39994 - 1188 Protein_45 ISL3 family transposase -
NV397_RS25735 (NV397_25735) 40112..40561 - 450 WP_105910642.1 AfaD family invasin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / dfrA12 / aadA2 / qacE cseA / iucA / iucB / iucC / iucD / iutA 1..186106 186106


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T254276 WP_059065587.1 NZ_CP102842:35409-35741 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT254276 WP_032454911.1 NZ_CP102842:35164-35409 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References