Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5206273..5206898 Replicon chromosome
Accession NZ_CP102841
Organism Klebsiella pneumoniae strain KP4863

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NV397_RS25140 Protein ID WP_002882817.1
Coordinates 5206273..5206656 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NV397_RS25145 Protein ID WP_004150355.1
Coordinates 5206656..5206898 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV397_RS25125 (NV397_25125) 5203639..5204541 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NV397_RS25130 (NV397_25130) 5204538..5205173 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NV397_RS25135 (NV397_25135) 5205170..5206099 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
NV397_RS25140 (NV397_25140) 5206273..5206656 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NV397_RS25145 (NV397_25145) 5206656..5206898 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NV397_RS25150 (NV397_25150) 5207103..5208020 + 918 WP_009309725.1 alpha/beta hydrolase -
NV397_RS25155 (NV397_25155) 5208034..5208975 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NV397_RS25160 (NV397_25160) 5209020..5209457 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NV397_RS25165 (NV397_25165) 5209454..5210314 - 861 WP_004146232.1 virulence factor BrkB family protein -
NV397_RS25170 (NV397_25170) 5210308..5210907 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254275 WP_002882817.1 NZ_CP102841:c5206656-5206273 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254275 WP_004150355.1 NZ_CP102841:c5206898-5206656 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References