Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 3685772..3686655 Replicon chromosome
Accession NC_015733
Organism Pseudomonas putida S16

Toxin (Protein)


Gene name - Uniprot ID A0A0C1HTG7
Locus tag PPS_RS16930 Protein ID WP_015270851.1
Coordinates 3685772..3686209 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID F8G488
Locus tag PPS_RS16935 Protein ID WP_013973163.1
Coordinates 3686206..3686655 (-) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPS_RS16915 3682142..3683371 - 1230 WP_013973159.1 acyl-CoA dehydrogenase -
PPS_RS16920 3683512..3684438 + 927 WP_013973160.1 LysR family transcriptional regulator -
PPS_RS16925 3684610..3685764 + 1155 WP_013973161.1 aminotransferase class V-fold PLP-dependent enzyme -
PPS_RS16930 3685772..3686209 - 438 WP_015270851.1 RES family NAD+ phosphorylase Toxin
PPS_RS16935 3686206..3686655 - 450 WP_013973163.1 DUF2384 domain-containing protein Antitoxin
PPS_RS16940 3686754..3687407 - 654 WP_013973164.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
PPS_RS16945 3687512..3688435 + 924 WP_013973165.1 LysR family transcriptional regulator -
PPS_RS16950 3688483..3689313 + 831 WP_013973166.1 AraC family transcriptional regulator -
PPS_RS16955 3689364..3689948 + 585 WP_013973167.1 LysE family translocator -
PPS_RS16960 3690069..3691286 - 1218 WP_044047087.1 sugar transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-137)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15969.19 Da        Isoelectric Point: 5.1199

>T25427 WP_015270851.1 NC_015733:c3686209-3685772 [Pseudomonas putida S16]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSSQTELTRGLGNRFLDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Download         Length: 438 bp

>T25427 NC_015733:c3686209-3685772 [Pseudomonas putida S16]
GTGATTCTGTGGCGTATCAGCGCCTATGCAGACTTGAGCGGAACGGGCGGCTTGCGTGTCAGCGGCCGCTGGCACCAGGC
GGGCCGGCCAGTGGTGTATGCCGCCACCAGCCCGGCCGGGGCGATGCTGGAGGTGCTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCTGACACCGTTTCGCAAGCGCAGTTGCCCGCCTTGCAG
CCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGCTTTCTGGATGAGTGTTCGGCGTTGCTGCTGCC
GGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTTTTCAACCCGCGCCATCCGCAGGCAAACAGTGCCAGGCTCG
AGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 17004.59 Da        Isoelectric Point: 6.3405

>AT25427 WP_013973163.1 NC_015733:c3686655-3686206 [Pseudomonas putida S16]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Download         Length: 450 bp

>AT25427 NC_015733:c3686655-3686206 [Pseudomonas putida S16]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCCTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGCGACCGGCTGTTCCGTTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCAGGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTAACCACCCAGCAGGGCACCA
CCCAGGTCGAAGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C1HTG7

Antitoxin

Source ID Structure
AlphaFold DB F8G488

References