Detailed information of TA system TA025426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2320610..2321126
• Replicon: chromosome
• Accession: NC_015733
• Organism: Pseudomonas putida S16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: PPS_RS10765
• Protein ID: WP_003259987.1
• Coordinates: 2320845..2321126 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: PPS_RS10760
• Protein ID: WP_013972043.1
• Coordinates: 2320610..2320855 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPS_RS10740	2316037..2316579	-	543	WP_013972039.1	WYL domain-containing protein	-
PPS_RS10745	2316687..2317673	-	987	WP_013972040.1	zinc-binding dehydrogenase	-
PPS_RS10750	2318036..2319229	+	1194	WP_013972041.1	MFS transporter	-
PPS_RS10755	2319260..2320366	+	1107	WP_031325095.1	alkene reductase	-
PPS_RS10760	2320610..2320855	+	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PPS_RS10765	2320845..2321126	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPS_RS10770	2321165..2321560	-	396	WP_023662326.1	hypothetical protein	-
PPS_RS10775	2321649..2322557	-	909	WP_013972045.1	LysR family transcriptional regulator	-
PPS_RS10780	2322849..2324153	+	1305	WP_013972046.1	MHS family MFS transporter	-
PPS_RS10785	2324184..2325425	+	1242	WP_013972047.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T25426 WP_003259987.1 NC_015733:2320845-2321126 [Pseudomonas putida S16]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T25426 NC_015733:2320845-2321126 [Pseudomonas putida S16]
ATGACCTATAAACTCGAATTCCTGCCTTCGGCACTGAAAGAGTGGGGCAAGCTAGGCCACACCGTGCGCGAACAGATCAA
GAAAAAACTCGCCGAGCGCCTGCAGTCACCCAAGGTACAGGCCGACGCGCTGCGTGACCTGCCGAACCACTACAAAATCA
AGCTCAAGGCCTCTGGATATCGCCTCGTCTATCGAGTCGAGGACGAGCGGATCGTTGTAGTGGTGGTATCGGTTGGCAAA
CGCGAGCGTAGCGAGGTGTATCGATCCGCTCAGAAGCGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT25426 WP_013972043.1 NC_015733:2320610-2320855 [Pseudomonas putida S16]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT25426 NC_015733:2320610-2320855 [Pseudomonas putida S16]
ATGCAAAACATATTCGCTGACGTGGCCGTAAGCGTGTCGGAACTGAAAAAGAACCCATCCGCGGTGATGACCGGCGCTGC
GGGTATGCCCGTCGCCGTGCTCAATCACAACCGCGTGATGGGTTACATGGTGCCGGCCGAACTGTACGAGGCCATGATGG
AACGCTTGGAAGATCTCGAATTGAGCGAACTGGCCAAGTCGAGGTTGGGTGAAAAAGGCGTACCGGTATCCCTGGATGAC
CTATAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6