Detailed information of TA system TA025425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3018547..3019112
• Replicon: chromosome
• Accession: NC_015732
• Organism: Treponema caldaria DSM 7334

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F8EZN8
• Locus tag: SPICA_RS13505
• Protein ID: WP_013970040.1
• Coordinates: 3018547..3018885 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F8EZN9
• Locus tag: SPICA_RS13510
• Protein ID: WP_013970041.1
• Coordinates: 3018879..3019112 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPICA_RS13475	3014644..3015795	-	1152	WP_156789803.1	BMP family ABC transporter substrate-binding protein	-
SPICA_RS13485	3016185..3016958	+	774	WP_013970036.1	hypothetical protein	-
SPICA_RS13490	3016995..3017390	-	396	WP_013970037.1	type II toxin-antitoxin system VapC family toxin	-
SPICA_RS13495	3017387..3017626	-	240	WP_013970038.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SPICA_RS13500	3017676..3018164	-	489	WP_156789689.1	hypothetical protein	-
SPICA_RS13505	3018547..3018885	-	339	WP_013970040.1	endoribonuclease MazF	Toxin
SPICA_RS13510	3018879..3019112	-	234	WP_013970041.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SPICA_RS13515	3019350..3019928	-	579	WP_013970042.1	hypothetical protein	-
SPICA_RS13520	3020736..3021443	-	708	WP_013970043.1	RluA family pseudouridine synthase	-
SPICA_RS13525	3021440..3022384	-	945	WP_013970044.1	Cof-type HAD-IIB family hydrolase	-
SPICA_RS13530	3022420..3023730	-	1311	WP_013970045.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13006.21 Da
• Isoelectric Point: 8.9662

>T25425 WP_013970040.1 NC_015732:c3018885-3018547 [Treponema caldarium DSM 7334]
MVKTTYIPNKGDIVWLDFNPQLGHEQRGRRPALVLSYKAYNEKIGLGIFCPITSKEKGYPFEVKIEGKKIKGCVLSDQLK
NLDWRIRNIEYIEKVNNEIVDEVIDKIKVIIE

Nucleotide

• Length: 339 bp

>T25425 NC_015732:c3018885-3018547 [Treponema caldarium DSM 7334]
ATGGTAAAAACAACATATATTCCAAATAAAGGCGATATAGTTTGGCTTGATTTTAATCCACAGTTAGGGCATGAACAGCG
TGGACGCAGACCTGCTTTAGTTCTATCGTATAAAGCATATAATGAAAAAATTGGATTAGGTATTTTTTGCCCTATTACAA
GCAAAGAAAAAGGATATCCATTTGAAGTTAAAATAGAAGGGAAAAAAATAAAAGGATGTGTGCTGAGTGATCAACTTAAA
AACTTAGATTGGAGAATTAGAAATATTGAATATATAGAAAAAGTTAACAATGAAATAGTTGATGAAGTAATAGATAAAAT
TAAAGTAATAATAGAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8595.81 Da
• Isoelectric Point: 4.5782

>AT25425 WP_013970041.1 NC_015732:c3019112-3018879 [Treponema caldarium DSM 7334]
MQTVVQKWGNSLGIRIPALYSKDLELKNGSTVEISEEEGKLVIIPKRISLNELLAKVTTENIHEAIETGFSVGNEEW

Nucleotide

• Length: 234 bp

>AT25425 NC_015732:c3019112-3018879 [Treponema caldarium DSM 7334]
ATGCAAACTGTTGTACAAAAATGGGGAAATAGCTTAGGAATTAGAATCCCAGCATTATATTCCAAGGATTTGGAACTAAA
AAATGGCAGTACAGTCGAAATATCGGAAGAAGAAGGAAAATTAGTTATTATACCAAAAAGAATTAGCTTAAATGAGTTAT
TGGCTAAAGTGACAACTGAAAATATTCATGAAGCAATAGAAACTGGTTTTTCAGTTGGAAATGAAGAATGGTAA

Structures

Toxin

• AlphaFold DB: F8EZN8

Antitoxin

• AlphaFold DB: F8EZN9