Detailed information of TA system TA025424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 778352..778991
• Replicon: chromosome
• Accession: NC_015732
• Organism: Treponema caldaria DSM 7334

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8EYS0
• Locus tag: SPICA_RS03590
• Protein ID: WP_013968178.1
• Coordinates: 778596..778991 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8EYR9
• Locus tag: SPICA_RS03585
• Protein ID: WP_013968177.1
• Coordinates: 778352..778609 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPICA_RS03570	774675..775322	-	648	WP_013968174.1	peroxiredoxin	-
SPICA_RS03575	775534..777036	+	1503	WP_013968175.1	sigma 54-interacting transcriptional regulator	-
SPICA_RS03580	777019..778218	-	1200	WP_013968176.1	acetate/propionate family kinase	-
SPICA_RS03585	778352..778609	+	258	WP_013968177.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SPICA_RS03590	778596..778991	+	396	WP_013968178.1	type II toxin-antitoxin system VapC family toxin	Toxin
SPICA_RS03595	779227..780510	+	1284	WP_013968179.1	sugar ABC transporter substrate-binding protein	-
SPICA_RS03600	780621..781532	+	912	WP_013968180.1	sugar ABC transporter permease	-
SPICA_RS03605	781534..782379	+	846	WP_013968181.1	carbohydrate ABC transporter permease	-
SPICA_RS03610	782384..783877	+	1494	WP_013968182.1	alpha-N-arabinofuranosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (2-78)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 13959.02 Da
• Isoelectric Point: 4.8674

>T25424 WP_013968178.1 NC_015732:778596-778991 [Treponema caldarium DSM 7334]
MVVDSSAILAILLGESDADLYIDALCSGERLLISAVNHLEVAIVIEARKGAIGGESLHSLMTHSGIEVLPLDASQAEIAL
AAWRTYGKGRHPAALNLGDCAAYALAKTLNQPLLYKGDDFSKTDIVAWKER

Nucleotide

• Length: 396 bp

>T25424 NC_015732:778596-778991 [Treponema caldarium DSM 7334]
ATGGTAGTAGATAGCTCAGCAATCTTGGCAATTCTCCTGGGAGAATCCGATGCAGATCTCTATATCGATGCACTTTGTTC
AGGTGAACGGCTCCTCATAAGTGCGGTGAACCATTTGGAAGTCGCAATCGTAATTGAAGCCCGAAAGGGTGCCATCGGTG
GAGAGAGCTTACATAGTTTGATGACCCACTCCGGCATTGAGGTACTGCCCCTCGATGCAAGTCAGGCAGAAATTGCCCTT
GCTGCATGGCGTACCTACGGGAAGGGCCGGCATCCTGCGGCCCTGAATCTGGGGGATTGCGCGGCCTATGCTCTAGCAAA
AACGCTTAACCAGCCCTTGTTGTACAAGGGTGATGATTTTTCTAAAACCGATATCGTTGCCTGGAAGGAGCGATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9498.94 Da
• Isoelectric Point: 5.8872

>AT25424 WP_013968177.1 NC_015732:778352-778609 [Treponema caldarium DSM 7334]
MISIRNAEVERLARELATAEGKSMTETILEALQRLKTEAAGEGERKKVILKEIARTCALAPDLDLRQPEKILGYDDIGVF
HYGSR

Nucleotide

• Length: 258 bp

>AT25424 NC_015732:778352-778609 [Treponema caldarium DSM 7334]
ATGATTAGTATCAGAAACGCAGAGGTGGAACGTCTTGCCCGGGAGTTAGCCACTGCGGAGGGTAAGTCCATGACTGAAAC
GATATTGGAAGCACTACAGCGATTAAAAACCGAAGCCGCTGGTGAAGGGGAACGAAAAAAGGTGATACTCAAGGAAATTG
CCCGGACCTGTGCTCTGGCTCCCGATCTGGATCTGCGCCAGCCGGAAAAAATACTTGGCTACGATGATATAGGTGTTTTT
CATTATGGTAGTAGATAG

Structures

Toxin

• AlphaFold DB: F8EYS0

Antitoxin

• AlphaFold DB: F8EYR9