Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5353616..5354241 Replicon chromosome
Accession NZ_CP102833
Organism Klebsiella pneumoniae strain KP4962

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NV394_RS26160 Protein ID WP_002882817.1
Coordinates 5353616..5353999 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NV394_RS26165 Protein ID WP_004150355.1
Coordinates 5353999..5354241 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV394_RS26145 (5350982) 5350982..5351884 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
NV394_RS26150 (5351881) 5351881..5352516 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
NV394_RS26155 (5352513) 5352513..5353442 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NV394_RS26160 (5353616) 5353616..5353999 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NV394_RS26165 (5353999) 5353999..5354241 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NV394_RS26170 (5354446) 5354446..5355363 + 918 WP_004901674.1 alpha/beta hydrolase -
NV394_RS26175 (5355377) 5355377..5356318 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NV394_RS26180 (5356363) 5356363..5356800 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NV394_RS26185 (5356797) 5356797..5357657 - 861 WP_002882807.1 virulence factor BrkB family protein -
NV394_RS26190 (5357651) 5357651..5358250 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254234 WP_002882817.1 NZ_CP102833:c5353999-5353616 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254234 WP_004150355.1 NZ_CP102833:c5354241-5353999 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References