Detailed information of TA system TA025423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 309039..309664
• Replicon: chromosome
• Accession: NC_015732
• Organism: Treponema caldaria DSM 7334

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8EZT3
• Locus tag: SPICA_RS01405
• Protein ID: WP_013967759.1
• Coordinates: 309269..309664 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8EZT2
• Locus tag: SPICA_RS01400
• Protein ID: WP_013967758.1
• Coordinates: 309039..309269 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPICA_RS01380	304955..306187	-	1233	WP_013967755.1	ribose-phosphate diphosphokinase	-
SPICA_RS01385	306299..307504	-	1206	WP_013967756.1	OmpA family protein	-
SPICA_RS01390	307675..307950	-	276	WP_052296314.1	type II toxin-antitoxin system VapC family toxin	-
SPICA_RS01395	307947..308192	-	246	WP_013967757.1	hypothetical protein	-
SPICA_RS01400	309039..309269	+	231	WP_013967758.1	antitoxin	Antitoxin
SPICA_RS01405	309269..309664	+	396	WP_013967759.1	type II toxin-antitoxin system VapC family toxin	Toxin
SPICA_RS01420	310245..310472	-	228	WP_041396100.1	hypothetical protein	-
SPICA_RS01425	310529..310987	-	459	WP_013967761.1	CIA30 family protein	-
SPICA_RS15155	311088..312550	+	1463	Protein_281	IS5 family transposase	-
SPICA_RS01440	312624..313664	+	1041	WP_013967762.1	DUF2804 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14993.34 Da
• Isoelectric Point: 5.2768

>T25423 WP_013967759.1 NC_015732:309269-309664 [Treponema caldarium DSM 7334]
MYLLDTNICIYIINNNPRVVVEKIKQLKPSQVKLSAISVGELEYGVCKSRNRERNKIALVDFVSGFDILPFDDADAEVFG
IVRAELEKRGQVIGSYDMQIAAQAIARDLILVTNNTREFERIKDLKFENWI

Nucleotide

• Length: 396 bp

>T25423 NC_015732:309269-309664 [Treponema caldarium DSM 7334]
ATGTATCTGTTGGACACAAACATCTGCATATATATCATAAATAACAATCCAAGAGTTGTTGTTGAAAAAATTAAACAGCT
AAAACCTTCACAGGTGAAATTATCCGCTATATCGGTTGGTGAACTTGAGTATGGTGTATGTAAAAGTAGAAACAGAGAAC
GAAATAAAATCGCCCTTGTCGACTTTGTTTCCGGATTTGATATTTTACCCTTTGATGATGCTGATGCGGAAGTATTTGGA
ATAGTCAGAGCAGAATTAGAAAAAAGAGGACAAGTAATCGGATCTTATGATATGCAAATTGCAGCACAGGCCATTGCACG
GGATTTAATTTTAGTTACAAACAACACCAGAGAATTTGAAAGAATTAAAGATTTGAAATTTGAAAATTGGATTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8811.06 Da
• Isoelectric Point: 5.0651

>AT25423 WP_013967758.1 NC_015732:309039-309269 [Treponema caldarium DSM 7334]
MLSTKVFMSGNSQAVRLPKEYQVDDKELYIKKIGSTIILFPKKNPWEAFERSLSEFTDDFMVDGRNQPPVQERDGL

Nucleotide

• Length: 231 bp

>AT25423 NC_015732:309039-309269 [Treponema caldarium DSM 7334]
ATGCTAAGCACTAAAGTTTTTATGAGTGGTAACAGTCAAGCGGTACGTCTCCCCAAAGAATATCAAGTTGATGATAAGGA
GCTGTACATAAAGAAAATAGGAAGCACTATTATTCTTTTCCCTAAAAAAAATCCTTGGGAAGCTTTTGAAAGGAGTCTTT
CAGAATTTACAGATGATTTTATGGTCGATGGGAGGAATCAGCCCCCTGTTCAGGAGCGAGATGGCTTATAA

Structures

Toxin

• AlphaFold DB: F8EZT3

Antitoxin

• AlphaFold DB: F8EZT2