Detailed information of TA system TA025422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 131534..132159
• Replicon: chromosome
• Accession: NC_015732
• Organism: Treponema caldaria DSM 7334

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8EYB8
• Locus tag: SPICA_RS00530
• Protein ID: WP_013967590.1
• Coordinates: 131534..131929 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8EYB9
• Locus tag: SPICA_RS00535
• Protein ID: WP_013967591.1
• Coordinates: 131929..132159 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPICA_RS00520	129487..129681	-	195	WP_013967588.1	hypothetical protein	-
SPICA_RS00525	130072..131448	+	1377	WP_013967589.1	ATP-binding protein	-
SPICA_RS00530	131534..131929	-	396	WP_013967590.1	type II toxin-antitoxin system VapC family toxin	Toxin
SPICA_RS00535	131929..132159	-	231	WP_013967591.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SPICA_RS00540	132352..133719	+	1368	WP_013967592.1	transglutaminase	-
SPICA_RS14560	133721..135736	+	2016	WP_013967593.1	hypothetical protein	-
SPICA_RS00550	135736..136488	+	753	WP_013967594.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	118177..132159	13982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15169.62 Da
• Isoelectric Point: 7.2756

>T25422 WP_013967590.1 NC_015732:c131929-131534 [Treponema caldarium DSM 7334]
MYLLDTNICIFIKNKKPKQVLDHLHQVINQNLYISSITVAELYFGVFNSQNIEKNHISLVEFLAPFKILDFDDEDAKEFG
RIRSKLKKEGKLIGPYDMQIAAQAIAKKLILVTNNTNEFIRVDGIKIEDWN

Nucleotide

• Length: 396 bp

>T25422 NC_015732:c131929-131534 [Treponema caldarium DSM 7334]
ATGTATCTACTCGATACAAATATTTGCATTTTTATAAAGAACAAAAAACCGAAACAGGTATTAGATCATTTACATCAAGT
AATTAACCAGAATTTATATATATCAAGTATTACAGTTGCTGAATTGTATTTTGGAGTTTTTAATAGTCAAAACATTGAAA
AAAACCATATTTCTTTAGTTGAGTTTTTAGCACCTTTTAAAATATTGGATTTTGATGATGAAGATGCAAAAGAATTTGGG
AGAATAAGATCAAAATTAAAGAAAGAAGGGAAGCTAATAGGTCCATATGATATGCAAATAGCGGCACAGGCAATAGCTAA
GAAATTAATCCTTGTTACAAATAATACAAATGAATTCATCAGAGTAGATGGAATCAAAATTGAAGATTGGAATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8720.90 Da
• Isoelectric Point: 4.7405

>AT25422 WP_013967591.1 NC_015732:c132159-131929 [Treponema caldarium DSM 7334]
MQTAKLFQNGRSQAVRLPKEYNFKGNDVLIQKVGEAVILFPKDRVWETFLEGLNGFTEDFMADGREQPAMQERDGL

Nucleotide

• Length: 231 bp

>AT25422 NC_015732:c132159-131929 [Treponema caldarium DSM 7334]
ATGCAAACCGCTAAACTATTCCAGAATGGCCGTAGTCAGGCAGTAAGACTACCGAAAGAATATAACTTCAAAGGTAATGA
TGTTCTTATCCAGAAGGTTGGAGAGGCTGTGATTCTATTCCCCAAAGATCGTGTTTGGGAGACTTTTCTTGAAGGTTTAA
ATGGATTCACAGAAGACTTTATGGCTGATGGAAGAGAACAACCAGCTATGCAAGAAAGAGATGGTTTATAA

Structures

Toxin

• AlphaFold DB: F8EYB8

Antitoxin

• AlphaFold DB: F8EYB9