Detailed information of TA system TA025421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 3672382..3673034
• Replicon: chromosome
• Accession: NC_015730
• Organism: Roseobacter litoralis Och 149

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RLO149_RS17580
• Protein ID: WP_148264393.1
• Coordinates: 3672621..3673034 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7ZB12
• Locus tag: RLO149_RS17575
• Protein ID: WP_013963445.1
• Coordinates: 3672382..3672624 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLO149_RS17555	3667613..3669988	+	2376	WP_148264500.1	HAMP domain-containing protein	-
RLO149_RS17565	3670735..3671908	+	1174	Protein_3457	IS3 family transposase	-
RLO149_RS17570	3671990..3672295	+	306	WP_013963444.1	hypothetical protein	-
RLO149_RS17575	3672382..3672624	+	243	WP_013963445.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RLO149_RS17580	3672621..3673034	+	414	WP_148264393.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLO149_RS17585	3673377..3673748	+	372	WP_013963447.1	GFA family protein	-
RLO149_RS17590	3673988..3675346	-	1359	WP_013963448.1	ISKra4-like element ISRli1 family transposase	-
RLO149_RS22950	3675532..3676164	-	633	WP_013963449.1	GNAT family N-acetyltransferase	-
RLO149_RS17600	3676498..3677520	+	1023	WP_148264501.1	autoinducer binding domain-containing protein	-
RLO149_RS17605	3677732..3677941	-	210	Protein_3465	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3671309..3729435	58126
	-	inside	IScluster/Tn	-	-	3670735..3675346	4611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-49)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15138.20 Da
• Isoelectric Point: 5.8818

>T25421 WP_148264393.1 NC_015730:3672621-3673034 [Roseobacter litoralis Och 149]
VRIILDTNVISGLRKPAQYPEVASWLRAQRETDLFLSAITLGEIERGVVLQEKKNPEFARALRTWLNATELQFSDRILEF
SARDARTWGALSATLGHAGADLMIAATALNNDARVATRNTTDFIPTGVSVVNPFDQL

Nucleotide

• Length: 414 bp

>T25421 NC_015730:3672621-3673034 [Roseobacter litoralis Och 149]
GTGAGAATAATCCTCGACACGAATGTAATCTCCGGTTTGCGAAAACCGGCTCAATATCCAGAAGTTGCGAGTTGGTTGAG
GGCTCAGCGAGAAACTGATCTCTTCCTGAGCGCCATTACGCTAGGAGAGATCGAGCGGGGAGTCGTTCTTCAGGAAAAGA
AAAACCCAGAATTCGCCCGAGCTCTCAGAACTTGGCTAAACGCCACTGAGTTGCAGTTCTCAGACCGGATCCTCGAGTTC
TCGGCCCGAGATGCGCGAACTTGGGGCGCACTCTCCGCCACATTAGGCCATGCAGGCGCAGATTTAATGATCGCCGCAAC
TGCCCTCAACAATGACGCAAGAGTTGCGACGCGTAACACAACCGATTTCATCCCAACTGGGGTGTCGGTTGTAAACCCAT
TCGATCAGCTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9052.22 Da
• Isoelectric Point: 6.9792

>AT25421 WP_013963445.1 NC_015730:3672382-3672624 [Roseobacter litoralis Och 149]
MWTLQDAKNKFSSVVEEALAGRPQEVTKRGKLAVVILSASEYARLQRAAADNRGTFKDHLLSFPGDDFERLQSCPRDVEW

Nucleotide

• Length: 243 bp

>AT25421 NC_015730:3672382-3672624 [Roseobacter litoralis Och 149]
ATGTGGACACTGCAGGACGCAAAGAACAAATTCAGTTCGGTTGTCGAAGAAGCGCTGGCTGGTCGACCACAAGAAGTTAC
GAAGCGCGGGAAACTAGCAGTCGTGATCTTGTCTGCCAGCGAGTACGCACGCTTGCAACGTGCTGCTGCAGACAATCGAG
GCACATTCAAGGATCATCTTCTCAGTTTCCCCGGTGATGACTTCGAACGGTTGCAGTCTTGCCCGCGAGACGTTGAGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: F7ZB12