Detailed information of TA system TA025420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 845565..846130
• Replicon: plasmid BB1p
• Accession: NC_015727
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8GU31
• Locus tag: CNE_RS33920
• Protein ID: WP_013959269.1
• Coordinates: 845759..846130 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8GU30
• Locus tag: CNE_RS33915
• Protein ID: WP_013959268.1
• Coordinates: 845565..845762 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS33895	841649..842641	+	993	WP_013959264.1	hypothetical protein	-
CNE_RS33900	842798..843181	+	384	Protein_776	cation:dicarboxylase symporter family transporter	-
CNE_RS33905	843583..844227	+	645	Protein_777	DUF2384 domain-containing protein	-
CNE_RS33910	845195..845386	+	192	Protein_778	hypothetical protein	-
CNE_RS33915	845565..845762	+	198	WP_013959268.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CNE_RS33920	845759..846130	+	372	WP_013959269.1	type II toxin-antitoxin system VapC family toxin	Toxin
CNE_RS38815	846127..846618	-	492	WP_049800725.1	helix-turn-helix domain-containing protein	-
CNE_RS33930	847261..849924	+	2664	WP_041229061.1	DEAD/DEAH box helicase	-
CNE_RS33935	849942..850964	+	1023	WP_013959271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1499175	1499175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13596.77 Da
• Isoelectric Point: 4.8728

>T25420 WP_013959269.1 NC_015727:845759-846130 [Cupriavidus necator N-1]
MILVDTSVWIDHINASDPVLISLLAEERVLAHPYVIGEISLGSLRDRDVVLGALLDLPRAPVVTPEETFYLIEREGLFNR
GIGYVDTSLLASARLQPGITIWTRDKRLKKVADELNLGAVLAH

Nucleotide

• Length: 372 bp

>T25420 NC_015727:845759-846130 [Cupriavidus necator N-1]
ATGATTCTTGTCGACACGTCGGTCTGGATTGACCACATCAACGCTTCTGATCCTGTGCTGATTAGCCTGCTTGCCGAGGA
GCGTGTGCTGGCTCATCCATATGTGATAGGCGAGATTTCGCTCGGTAGTCTGCGTGACCGTGACGTCGTGCTTGGCGCGT
TGCTCGATCTGCCGCGCGCGCCAGTTGTAACGCCGGAGGAGACGTTCTATCTGATCGAGCGCGAAGGTTTGTTCAATCGT
GGAATCGGATATGTCGACACGTCACTGTTGGCGTCCGCGCGCCTACAACCAGGCATCACCATCTGGACGCGCGACAAGCG
ATTGAAGAAAGTTGCCGATGAACTCAATCTCGGCGCGGTGCTCGCGCACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7087.22 Da
• Isoelectric Point: 10.8072

>AT25420 WP_013959268.1 NC_015727:845565-845762 [Cupriavidus necator N-1]
MRTTIALDDDLIAKAQAYTGLKEKTALVREALKALIQREAAKRLANLGGSQPGIKGAPRRRQDVE

Nucleotide

• Length: 198 bp

>AT25420 NC_015727:845565-845762 [Cupriavidus necator N-1]
ATGCGCACGACTATTGCGCTTGATGACGACTTGATCGCCAAGGCTCAAGCCTATACGGGCCTGAAGGAAAAAACGGCACT
CGTGCGCGAAGCGCTAAAGGCCTTGATCCAGCGCGAAGCCGCGAAACGACTCGCGAATCTTGGCGGTAGTCAACCGGGCA
TCAAGGGGGCGCCGCGTCGCCGGCAGGACGTCGAATGA

Structures

Toxin

• AlphaFold DB: F8GU31

Antitoxin

• AlphaFold DB: F8GU30