Detailed information of TA system TA025419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 773932..774968
• Replicon: plasmid BB1p
• Accession: NC_015727
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CNE_RS33590
• Protein ID: WP_013959204.1
• Coordinates: 774396..774968 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: F8GXU6
• Locus tag: CNE_RS33585
• Protein ID: WP_012354456.1
• Coordinates: 773932..774393 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS33560	769113..769763	-	651	WP_063712361.1	DJ-1/PfpI family protein	-
CNE_RS33565	769843..770544	-	702	WP_148271766.1	XRE family transcriptional regulator	-
CNE_RS33570	770690..772030	-	1341	WP_013959201.1	MHS family MFS transporter	-
CNE_RS33575	772291..772707	+	417	WP_013959202.1	cupin domain-containing protein	-
CNE_RS33580	772770..773090	+	321	WP_041229052.1	ferredoxin family protein	-
CNE_RS33585	773932..774393	+	462	WP_012354456.1	excisionase family DNA-binding protein	Antitoxin
CNE_RS33590	774396..774968	+	573	WP_013959204.1	PIN domain-containing protein	Toxin
CNE_RS40035	775041..775154	+	114	Protein_711	H-NS histone family protein	-
CNE_RS33595	775313..775681	+	369	WP_013959205.1	calcium-binding protein	-
CNE_RS33600	775913..777691	+	1779	WP_013959206.1	hypothetical protein	-
CNE_RS33605	778068..779498	+	1431	WP_041229053.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1499175	1499175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Helix-turn-helix domain, group 17 (83-130)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21230.22 Da
• Isoelectric Point: 5.0423

>T25419 WP_013959204.1 NC_015727:774396-774968 [Cupriavidus necator N-1]
MTSNFAVVYDACALYPAPLRDLLMRLALTDLYRAKWANQIHDEWIRNLLARRPELSADRLAGTRELMNRSVRDSLVSGYE
HLIESIELPDPDDRHVVAAAIHSGSEAIITFNMKDFPETALKEFNVEAIHPDDFVVDLFDLNAGKVLAAVAEHRASLKNP
PKSPSEYLTTLSAQGLTQTVLILTQYSLAI

Nucleotide

• Length: 573 bp

>T25419 NC_015727:774396-774968 [Cupriavidus necator N-1]
ATGACTTCCAACTTCGCTGTAGTCTACGATGCCTGCGCTCTCTATCCTGCTCCGCTGCGCGATCTGCTGATGCGGCTCGC
GCTGACCGACCTGTACCGTGCAAAGTGGGCGAATCAAATTCATGACGAGTGGATTCGCAACCTCCTTGCGCGGCGGCCGG
AGCTTTCCGCGGATCGCCTTGCAGGCACGCGCGAACTGATGAACCGAAGTGTTCGCGATTCGCTGGTTAGCGGCTATGAG
CACCTTATTGAGTCCATTGAGTTGCCTGATCCTGATGATCGACATGTGGTTGCGGCCGCAATTCATAGCGGCTCGGAGGC
AATCATCACGTTCAATATGAAGGACTTCCCGGAAACCGCACTCAAGGAGTTCAACGTCGAGGCGATCCACCCGGACGATT
TCGTCGTGGACCTCTTTGATCTGAATGCAGGGAAGGTCCTGGCGGCAGTGGCGGAACACCGTGCATCTCTGAAGAATCCA
CCGAAGTCTCCTTCGGAGTATTTGACTACCCTTTCGGCGCAAGGCCTGACGCAAACGGTGTTGATCCTAACGCAGTACAG
TCTGGCAATCTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17046.22 Da
• Isoelectric Point: 5.0133

>AT25419 WP_012354456.1 NC_015727:773932-774393 [Cupriavidus necator N-1]
MHATITNTTLPSADESAIARESSRSLAVALDSRSDTQQFDFKTDKGELHSVTLPTSALRLLVEVLAEIGQGNAVSIIPIH
AELTTQEAADLLNVSRPYLVQLLEKGEIPFRKVNTHRRVLYQDVVSYKQRIDDERRKALDELAAQAQELDMGY

Nucleotide

• Length: 462 bp

>AT25419 NC_015727:773932-774393 [Cupriavidus necator N-1]
ATGCACGCAACCATCACCAACACGACGCTGCCTTCGGCAGACGAAAGTGCTATCGCTCGGGAATCGAGCCGCAGCCTGGC
GGTGGCCTTGGACAGCCGATCCGATACGCAGCAATTCGATTTCAAGACTGACAAGGGCGAGTTGCATAGTGTCACATTGC
CCACCTCTGCGCTGAGGCTTCTCGTCGAGGTTCTCGCGGAAATCGGCCAAGGGAACGCCGTCTCCATCATTCCCATTCAC
GCCGAGCTGACGACGCAAGAGGCCGCGGACCTGCTTAACGTCTCTCGACCATACTTGGTTCAGCTTCTGGAGAAGGGCGA
GATTCCGTTCCGTAAAGTCAACACGCATCGGCGCGTCCTCTACCAGGACGTTGTCAGCTACAAGCAGCGCATTGATGACG
AGCGACGCAAGGCACTAGACGAACTCGCAGCGCAGGCCCAAGAGTTGGACATGGGCTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A375HZE9