Detailed information of TA system TA025417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 229750..230227
• Replicon: chromosome
• Accession: NC_015726
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: G0ETC7
• Locus tag: CNE_RS01145
• Protein ID: WP_013955321.1
• Coordinates: 229958..230227 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: G0ETC6
• Locus tag: CNE_RS01140
• Protein ID: WP_013955320.1
• Coordinates: 229750..229974 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS01115	225787..226980	+	1194	WP_013955316.1	FAD-binding oxidoreductase	-
CNE_RS01120	226993..227190	+	198	WP_041228272.1	sulfur carrier protein ThiS	-
CNE_RS01125	227194..228030	+	837	WP_010813326.1	thiazole synthase	-
CNE_RS01130	228027..229163	+	1137	WP_013955318.1	thiamine phosphate synthase	-
CNE_RS01135	229178..229696	+	519	WP_013955319.1	GNAT family N-acetyltransferase	-
CNE_RS01140	229750..229974	+	225	WP_013955320.1	TraY domain-containing protein	Antitoxin
CNE_RS01145	229958..230227	+	270	WP_013955321.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CNE_RS01150	230333..230785	+	453	WP_013955322.1	PaaI family thioesterase	-
CNE_RS01155	230807..231241	-	435	WP_013955323.1	PGDYG domain-containing protein	-
CNE_RS01160	231247..232074	-	828	WP_049800538.1	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-
CNE_RS01165	232371..233789	+	1419	WP_010813332.1	adenosylhomocysteinase	-
CNE_RS01170	233861..234211	+	351	WP_013955325.1	phage holin family protein	-
CNE_RS01175	234273..235103	+	831	WP_013955326.1	methylenetetrahydrofolate reductase [NAD(P)H]	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-48)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10401.99 Da
• Isoelectric Point: 10.0391

>T25417 WP_013955321.1 NC_015726:229958-230227 [Cupriavidus necator N-1]
MAWRIEVTQTAEKQLAKLDRPVARRIVTFLREQVAASDDPRSLGEALKGSELGEYWKYRVGDWRLICQIEDARITVVVLR
LGNRRDVYR

Nucleotide

• Length: 270 bp

>T25417 NC_015726:229958-230227 [Cupriavidus necator N-1]
TTGGCCTGGCGGATTGAAGTCACGCAAACGGCAGAGAAACAACTTGCCAAGCTGGATCGGCCGGTCGCCAGGCGTATTGT
CACGTTCTTGCGCGAGCAAGTCGCGGCATCCGACGACCCCCGCTCGCTTGGCGAAGCCCTGAAGGGGAGTGAGCTTGGCG
AATACTGGAAATACCGGGTCGGCGACTGGCGTTTGATTTGCCAGATCGAGGACGCGCGCATCACTGTTGTGGTGCTGCGT
CTTGGGAACCGCAGGGACGTGTATCGCTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8387.53 Da
• Isoelectric Point: 4.4066

>AT25417 WP_013955320.1 NC_015726:229750-229974 [Cupriavidus necator N-1]
MLAIRLPEDIEDRLEALAKRTGRTKTFYAREAIMQHLDDLEDLYLADKIAARVRAGEESTVTLDELEARLGLAD

Nucleotide

• Length: 225 bp

>AT25417 NC_015726:229750-229974 [Cupriavidus necator N-1]
ATGCTCGCTATCCGACTACCAGAGGACATCGAAGACCGCCTGGAAGCCCTTGCCAAACGCACCGGCCGCACCAAGACGTT
TTATGCGCGTGAGGCCATCATGCAGCACCTTGATGATCTTGAGGACCTGTATCTGGCAGACAAGATTGCCGCACGTGTTC
GCGCCGGCGAAGAATCCACGGTAACGCTGGATGAGCTGGAGGCGCGGCTTGGCCTGGCGGATTGA

Structures

Toxin

• AlphaFold DB: G0ETC7

Antitoxin

• AlphaFold DB: G0ETC6