Detailed information of TA system TA025416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2283159..2283802
• Replicon: chromosome
• Accession: NC_015723
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CNE_RS28390
• Protein ID: WP_013953738.1
• Coordinates: 2283159..2283560 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8GSK5
• Locus tag: CNE_RS28395
• Protein ID: WP_013953739.1
• Coordinates: 2283560..2283802 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS28375	2278435..2278920	+	486	WP_013953735.1	DM13 domain-containing protein	-
CNE_RS28380	2279094..2280164	-	1071	WP_041228713.1	porin	-
CNE_RS28385	2280475..2283102	+	2628	WP_013953736.1	transglycosylase domain-containing protein	-
CNE_RS28390	2283159..2283560	-	402	WP_013953738.1	type II toxin-antitoxin system VapC family toxin	Toxin
CNE_RS28395	2283560..2283802	-	243	WP_013953739.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CNE_RS28400	2283936..2285258	-	1323	WP_013953740.1	DUF1501 domain-containing protein	-
CNE_RS28405	2285272..2286861	-	1590	WP_013953741.1	DUF1800 domain-containing protein	-
CNE_RS28410	2287060..2287983	+	924	WP_013953743.1	DMT family transporter	-
CNE_RS28415	2287967..2288731	+	765	WP_013953744.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (13-48)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 13805.93 Da
• Isoelectric Point: 5.8330

>T25416 WP_013953738.1 NC_015723:c2283560-2283159 [Cupriavidus necator N-1]
MSLFLLDTNIISDAIRNTRGPCAARIAALEPDSLCTSIIVAAELRFGAAKKGSPALSQRVGQALAGLGVLPLGGDADACY
GELRAGLERSGRVIGANDMLIAAHALSLGAVLVTDNIGEFSRVDGLRCENWLK

Nucleotide

• Length: 402 bp

>T25416 NC_015723:c2283560-2283159 [Cupriavidus necator N-1]
ATGTCCTTGTTCCTGCTCGATACGAACATCATTTCCGACGCGATTCGCAATACCCGCGGACCCTGTGCCGCTCGTATTGC
CGCGCTCGAGCCTGACAGTCTGTGCACTTCCATCATTGTGGCGGCCGAACTCCGATTCGGAGCTGCCAAGAAAGGCTCTC
CGGCGCTGAGCCAACGTGTCGGCCAGGCTTTGGCTGGCTTGGGTGTCTTGCCGCTGGGCGGCGATGCTGACGCTTGCTAC
GGCGAGTTGCGCGCAGGACTGGAACGCAGCGGACGGGTGATTGGCGCCAATGACATGCTGATCGCGGCGCACGCTCTATC
GCTGGGTGCCGTGCTAGTGACCGACAATATCGGGGAGTTTTCTCGCGTGGACGGATTGCGCTGCGAAAACTGGCTGAAGT
AG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9281.58 Da
• Isoelectric Point: 4.4791

>AT25416 WP_013953739.1 NC_015723:c2283802-2283560 [Cupriavidus necator N-1]
MTTTDEIRHVRLFRNGRNQAIRIPREFELPGEEAILRREGNHLIIEPVAAMPLRSLLAQWEPIDVDWPTIDNLPAEDVDL

Nucleotide

• Length: 243 bp

>AT25416 NC_015723:c2283802-2283560 [Cupriavidus necator N-1]
ATGACCACGACAGATGAAATCCGCCATGTCAGGCTGTTTCGCAATGGCCGCAATCAGGCGATCCGGATTCCGCGCGAGTT
CGAACTGCCGGGGGAGGAAGCCATTCTGCGCCGCGAAGGGAATCACCTCATCATCGAGCCGGTTGCTGCAATGCCATTGC
GTTCCTTGCTGGCGCAATGGGAGCCTATCGACGTCGATTGGCCGACGATCGATAACCTGCCTGCCGAGGATGTCGACCTC
TGA

Structures

Antitoxin

• AlphaFold DB: F8GSK5