Detailed information of TA system TA025413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-TIGR02293
• Location: 1308171..1309156
• Replicon: chromosome
• Accession: NC_015723
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: F8GMR0
• Locus tag: CNE_RS24100
• Protein ID: WP_013952895.1
• Coordinates: 1308683..1309156 (+)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: F8GMQ9
• Locus tag: CNE_RS24095
• Protein ID: WP_013952894.1
• Coordinates: 1308171..1308686 (+)
• Length: 172 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS24065	1303604..1304248	+	645	WP_013952889.1	TetR/AcrR family transcriptional regulator	-
CNE_RS24070	1304563..1304880	+	318	WP_013952890.1	hypothetical protein	-
CNE_RS24075	1304958..1305140	+	183	WP_041228627.1	hypothetical protein	-
CNE_RS24080	1305192..1305617	-	426	WP_035823937.1	hypothetical protein	-
CNE_RS24085	1305867..1306994	-	1128	WP_013952892.1	site-specific integrase	-
CNE_RS24090	1307107..1308072	+	966	WP_013952893.1	DNA-binding protein	-
CNE_RS24095	1308171..1308686	+	516	WP_013952894.1	DUF2384 domain-containing protein	Antitoxin
CNE_RS24100	1308683..1309156	+	474	WP_013952895.1	RES family NAD+ phosphorylase	Toxin
CNE_RS24105	1309160..1310737	-	1578	WP_013952896.1	fatty acid--CoA ligase	-
CNE_RS24110	1310991..1313240	+	2250	WP_041228628.1	ATP-dependent DNA helicase	-
CNE_RS24115	1313367..1313690	+	324	WP_013952898.1	thioredoxin family protein	-
CNE_RS24120	1313708..1314139	-	432	WP_010809388.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (4-147)

Antitoxin

• Antitoxin Xre (36-171)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 16788.97 Da
• Isoelectric Point: 5.5721

>T25413 WP_013952895.1 NC_015723:1308683-1309156 [Cupriavidus necator N-1]
VRPAWRIATDTPDYTADDASGAGAKATGGRWNRQGTHLIYAASSIALACLETLVHLGAGGLPLNRYLVRIDIPDELWAAA
QQLSADTAPVGWDAIPAGKTSLDTGEAWTAEGTSALLLVPSIVIPEEHNVLVNPAHPDAARLAYVKLRRWQYDARLV

Nucleotide

• Length: 474 bp

>T25413 NC_015723:1308683-1309156 [Cupriavidus necator N-1]
GTGAGGCCAGCCTGGCGGATCGCGACCGACACCCCAGACTACACCGCGGACGATGCCAGCGGCGCCGGCGCCAAGGCCAC
CGGCGGGCGCTGGAACCGGCAGGGCACGCACTTGATCTATGCGGCGTCCAGCATCGCGCTGGCTTGCCTGGAAACGCTGG
TCCACCTGGGCGCGGGCGGGCTGCCACTGAACCGCTACCTGGTCCGGATCGACATCCCTGACGAACTATGGGCGGCGGCG
CAGCAACTGAGCGCGGACACCGCCCCCGTTGGATGGGATGCCATTCCCGCCGGCAAGACCAGCCTCGACACCGGCGAAGC
CTGGACCGCCGAGGGAACCAGCGCCTTGCTGCTGGTGCCCTCTATCGTCATCCCTGAAGAACACAACGTGCTGGTCAACC
CGGCACACCCCGACGCGGCGCGGCTGGCCTACGTCAAGCTGCGCCGCTGGCAGTATGACGCGCGCCTAGTCTGA

Antitoxin

Protein

• Length: 172 a.a.
• Molecular weight: 18542.04 Da
• Isoelectric Point: 7.2047

>AT25413 WP_013952894.1 NC_015723:1308171-1308686 [Cupriavidus necator N-1]
MPFTLTPEGRYPLAHERPSPPELSVNNYVAVYEASLSTQGAMSVIRAIREGVPASDLTRLAESMGTTKEQLIKTLDLPRA
TVDRKARAHQNLSSEQSERVLGMARLVGQVQALVESSGDPRGFSAAQWVAQWLEQPSPALGGRRPAELMDTLAGQRIVSD
LVARMQSGAYA

Nucleotide

• Length: 516 bp

>AT25413 NC_015723:1308171-1308686 [Cupriavidus necator N-1]
ATGCCGTTCACGCTCACTCCCGAAGGCCGCTATCCGCTGGCTCATGAACGCCCGTCACCGCCCGAGCTGAGCGTCAACAA
CTATGTCGCCGTCTATGAGGCCAGCCTGTCGACGCAAGGTGCCATGTCGGTCATCCGCGCGATTCGTGAAGGCGTGCCGG
CTTCAGACCTGACCCGCCTGGCCGAATCCATGGGCACGACCAAGGAGCAGCTGATCAAGACACTGGATTTGCCGCGCGCC
ACTGTGGACCGCAAGGCACGGGCGCACCAGAACCTGTCGAGCGAGCAGAGCGAGCGTGTGCTGGGCATGGCGCGGCTGGT
CGGGCAGGTCCAGGCGCTGGTGGAAAGCTCGGGCGATCCGCGCGGTTTCAGCGCGGCACAGTGGGTGGCGCAGTGGCTGG
AGCAGCCGTCGCCGGCGCTGGGCGGGCGCCGCCCCGCTGAGCTGATGGATACGCTCGCCGGCCAGCGCATCGTTTCCGAC
CTAGTGGCCAGGATGCAGAGCGGGGCGTACGCGTGA

Structures

Toxin

• AlphaFold DB: F8GMR0

Antitoxin

• AlphaFold DB: F8GMQ9