Detailed information of TA system TA025412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 711514..712193
• Replicon: chromosome
• Accession: NC_015723
• Organism: Cupriavidus necator N-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8GRR1
• Locus tag: CNE_RS21350
• Protein ID: WP_013952353.1
• Coordinates: 711765..712193 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CNE_RS21345
• Protein ID: WP_041228808.1
• Coordinates: 711514..711768 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNE_RS21325	707169..707630	-	462	WP_013952348.1	MaoC family dehydratase N-terminal domain-containing protein	-
CNE_RS21330	707627..708850	-	1224	WP_013952349.1	CoA transferase	-
CNE_RS21335	708957..709847	+	891	WP_013952350.1	LysR family transcriptional regulator	-
CNE_RS21340	709872..711068	-	1197	WP_013952351.1	helix-turn-helix transcriptional regulator	-
CNE_RS41060	711204..711515	-	312	WP_148271647.1	hypothetical protein	-
CNE_RS21345	711514..711768	+	255	WP_041228808.1	plasmid stabilization protein	Antitoxin
CNE_RS21350	711765..712193	+	429	WP_013952353.1	type II toxin-antitoxin system VapC family toxin	Toxin
CNE_RS21355	712221..714590	-	2370	WP_013952355.1	mechanosensitive ion channel family protein	-
CNE_RS21360	714855..715637	+	783	WP_013952356.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 14987.27 Da
• Isoelectric Point: 4.7961

>T25412 WP_013952353.1 NC_015723:711765-712193 [Cupriavidus necator N-1]
MIVLDTNVISEAMKPEPADSVRCWLNDQAAETLYLSSVTLAELLFGIGALPAGRRRAMLAAALDGLVTLFEGRILPFDTN
AARHYAELATAARSRGRGLPTPDGYIAAIAAANGMIVATRDVSPFEAAGLQVLNPWLAPPPS

Nucleotide

• Length: 429 bp

>T25412 NC_015723:711765-712193 [Cupriavidus necator N-1]
ATGATCGTCCTGGATACGAATGTCATTTCCGAGGCCATGAAGCCGGAACCGGCTGACTCCGTGCGCTGCTGGCTCAACGA
CCAGGCCGCCGAGACCTTGTACCTGTCCAGTGTGACGCTGGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCCGCGGGCA
GGCGCCGCGCCATGCTGGCCGCTGCGCTGGACGGCCTGGTGACGCTGTTCGAAGGGCGCATCTTGCCGTTCGACACGAAT
GCGGCCCGGCACTATGCGGAACTCGCCACTGCCGCCAGAAGCCGTGGCCGCGGCCTTCCCACGCCGGATGGCTACATCGC
CGCGATTGCCGCGGCCAACGGCATGATAGTCGCGACGCGGGATGTGTCGCCCTTTGAAGCGGCCGGGCTGCAGGTGCTCA
ACCCGTGGCTGGCGCCGCCTCCGTCGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9175.52 Da
• Isoelectric Point: 7.0159

>AT25412 WP_041228808.1 NC_015723:711514-711768 [Cupriavidus necator N-1]
MPILTVRNVPDDVHRALRVRAAQNGRSTEAEVREILASAVMPQKRVRLGDALAMLGREIGLSNADVEAIEQGKDKAAAEP
VRFE

Nucleotide

• Length: 255 bp

>AT25412 NC_015723:711514-711768 [Cupriavidus necator N-1]
ATGCCGATCCTGACCGTTAGAAATGTGCCCGACGACGTGCACCGTGCGCTGCGGGTCCGTGCCGCCCAGAACGGACGCAG
CACTGAAGCTGAGGTGCGTGAGATCCTGGCCAGCGCGGTGATGCCGCAGAAGCGCGTGCGCCTGGGCGATGCGCTGGCGA
TGCTTGGCCGCGAGATCGGCTTGTCGAATGCGGACGTTGAAGCGATCGAGCAGGGCAAGGACAAGGCAGCGGCCGAGCCG
GTGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: F8GRR1