Detailed information of TA system TA025411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 28982..29477
• Replicon: plasmid pZYMOP02
• Accession: NC_015716
• Organism: Zymomonas mobilis subsp. pomaceae ATCC 29192

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8EWK3
• Locus tag: ZYMOP_RS09150
• Protein ID: WP_013945653.1
• Coordinates: 28982..29227 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: F8EWK4
• Locus tag: ZYMOP_RS09155
• Protein ID: WP_013945654.1
• Coordinates: 29214..29477 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZYMOP_RS09110	24464..24739	+	276	WP_013945645.1	hypothetical protein	-
ZYMOP_RS09115	25422..25667	+	246	WP_049778898.1	helix-turn-helix domain-containing protein	-
ZYMOP_RS09125	27019..27339	-	321	WP_013945648.1	DUF1870 family protein	-
ZYMOP_RS09130	27348..27728	-	381	WP_013945649.1	type II toxin-antitoxin system death-on-curing family toxin	-
ZYMOP_RS09135	27725..27955	-	231	WP_013945650.1	hypothetical protein	-
ZYMOP_RS09140	28098..28280	-	183	WP_013945651.1	hypothetical protein	-
ZYMOP_RS09475	28465..28629	+	165	WP_013945652.1	hypothetical protein	-
ZYMOP_RS09480	28754..28900	-	147	WP_162094009.1	hypothetical protein	-
ZYMOP_RS09150	28982..29227	-	246	WP_013945653.1	type II toxin-antitoxin system YafQ family toxin	Toxin
ZYMOP_RS09155	29214..29477	-	264	WP_013945654.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ZYMOP_RS09160	30051..30503	-	453	WP_013945655.1	hypothetical protein	-
ZYMOP_RS09165	30614..31087	-	474	WP_013945656.1	helix-turn-helix domain-containing protein	-
ZYMOP_RS09170	31571..31888	+	318	WP_013945657.1	hypothetical protein	-
ZYMOP_RS09175	32013..32264	+	252	WP_013945658.1	DUF2312 domain-containing protein	-
ZYMOP_RS09180	32371..34059	+	1689	WP_013945659.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34161	34161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-80)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-81)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9665.18 Da
• Isoelectric Point: 9.5744

>T25411 WP_013945653.1 NC_015716:c29227-28982 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
MRMIERTSQFKRDYKRELKSDARLDKYFIPVITDLANDIALAPHLKDHPLTSNWNDHRECHIKPNLLLIYRKPNEGTLHL
V

Nucleotide

• Length: 246 bp

>T25411 NC_015716:c29227-28982 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
ATGCGGATGATTGAACGAACGTCTCAATTCAAACGCGATTATAAAAGAGAACTGAAGTCAGACGCACGATTAGATAAATA
TTTCATCCCTGTTATTACCGATCTAGCCAATGATATTGCGCTTGCTCCTCATTTGAAGGATCATCCGTTAACAAGTAATT
GGAATGATCATAGAGAATGCCATATTAAGCCTAATTTACTTTTGATTTATCGAAAGCCGAATGAAGGCACGCTTCACTTG
GTGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9492.11 Da
• Isoelectric Point: 9.8953

>AT25411 WP_013945654.1 NC_015716:c29477-29214 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
MAANQLVQARINGQIKQEASAILESIGLTVSDAVRMMLTKVVQEKALPFEPLVPNKKTLAAMKDARAGKLKKFNSVQSLM
DDLHADD

Nucleotide

• Length: 264 bp

>AT25411 NC_015716:c29477-29214 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
ATGGCAGCTAATCAACTCGTACAAGCTCGGATTAATGGACAGATAAAACAAGAAGCTTCTGCTATTTTGGAAAGCATTGG
TCTTACTGTATCCGATGCTGTCCGCATGATGCTAACAAAAGTCGTTCAAGAAAAAGCACTACCTTTTGAACCTCTTGTCC
CAAATAAAAAGACCTTGGCGGCGATGAAAGATGCGCGTGCCGGTAAATTAAAAAAATTCAACAGTGTTCAATCTCTGATG
GATGACCTTCATGCGGATGATTGA

Structures

Toxin

• AlphaFold DB: F8EWK3

Antitoxin

• AlphaFold DB: F8EWK4